Differential metabolites analysis on two varieties of fermented cigar leaves in semi-arid region
By comparing the metabolites between two varieties of fermented cigar leaves 'Haiyan 103 ' and'Qingxue 204',the metabolome differences between two varieties of cigar leaves were explored.In the study,non-targeted metabolomic analysis of the chemical components of two fermented cigar leaves were analyzed by Ultra High-Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometric (UHPLC-QTOF-MS).The results indicated that,the metabolomes relative content of'Haiyan 103' and 'Qingxue 204' were significantly different.A total of 179 differential metabolites were identified in two varieties of fermented cigar leaves,of which 114 metabolites were significantly up-regulated in 'Haiyan 103' compared to 'Qingxue 204',while 65 were sig-nificantly down-regulated.Forty major differential metabolites were qualitatively and quantitatively identified,inclu-ding 10 organic acids,7 amino acids,4 phenolic acids,4 nucleotides and analogues,3 carbohydrates,3 fla-vonoids,3 lignans and coumarins,and 6 others.Based on KEGG pathway analysis,12 significantly enriched path-ways were identified.
cigar varietiestobacconon-targeted metabolomicsdifferential metabolitessemi-arid region