To identify the alleviating effects of different alkaline soil amendments on soybean continuous crop-ping obstacles in black soil area,six different fertilization treatments including plot planting,namely no fertilization ( CK),conventional fertilization ( CF),phosphorus fertilizer reduction+calcium-magnesium-phosphorus fertilizer improver ( CPK),conventional fertilization+biochar ( CBK),conventional fertilization+quicklime ( CNK) and conventional fertilization+humic acid fertilizer ( CHK) were set up in 2023.No fertilization ( CK) and convention-al fertilization ( CF) were used as controls,and the physical and chemical properties of soil and the diversity and richness of soil microbial and bacterial communities were determined.The results showed that compared with the CK and CF treatments,the application of amendments increased soil organic carbon content by 3.9%~6.9%,soil total nitrogen content by 17.0%~41.0%,and available potassium content by 13.9%~21.8%.Compared with CK and CF,the soybean yield increased by 9.8%~32.1% under different amendment treatments,and the soybean yield in-creased the most under CBK treatment.The dominant phylum of soil bacteria was the same as those before improve-ment,but the richness and diversity of bacteria increased.Compared with the CK and CF controls,the relative abun-dance of Proteobacteria was increased by 10.6%~16.6% under the application of other modifiers except CNK treat-ment .The correlation between soil microbial diversity and soil physicochemical property showed that soil pH was posi-tively correlated with soil microbial alpha diversity index,while soil exchange acid content was negatively correlated with alpha diversity index.There was a significant negative correlation between soil available phosphorus content and soil Simpson index.In conclusion,amendments application optimized the physicochemical properties and microbial en-vironment of soybean continuous cropping soil and improved soybean yields.Of which CBK treatment had the best effect.
关键词
土壤改良剂/连作大豆/土壤酸化/土壤养分/细菌群落结构/微生物多样性
Key words
soil amendments/continuous cropping of soybean/soil acidification/soil nutrients/bacterial community structure/microbial diversity