Identification and evaluation of drought tolerance for 86 millet germplasms at mid to late growth stage in Inner Mongolia
Using 86 millet germplasms as research objects,field experiments were carried out in Chifeng City,Inner Mongolia in 2022 and 2023 to assess field drought tolerance during their mid to late growth stages.The study utilized two treatments of normal irrigation(CK)and drought stress(T).Nine morphological indicators,including plant height,stem basal diameter,spike stem length,spike length,spike diameter,SPAD chlorophyll content,single spike weight,1000-grain weight,and yield,were assessed.Comprehensive drought resistance coefficient(CDC),drought resistance index(D),and weighted drought resistance coefficient(WDC)were combined to identify the drought tolerance of millet at mid to late growth stage.The research results showed that under drought stress,all 9 measured indicators showed a decrease,with the order of decrease being yield(35.17%)>single spike weight(17.36%)>spike stem length(16.51%)>spike length(14.08%)>spike diameter(13.77%)>stem basal diameter(12.38%)>plant height(12.37%)>chlorophyll content(10.16%)>1000-grain weight(9.09%).Correlation analysis revealed the correlation between spike diameter and yield being the strongest,at a coefficient of 0.97.Principal component analysis transformed the nine evaluation indexes into five comprehensive in-dexes,accounting for a cumulative variance contribution of 89.2%.The 86 millet accessions were categorized into five groups based on CDC,D,WDC value cluster analysis.Three comprehensive evaluation methods for drought re-sistance identified 9 accessions in cluster I as highly drought tolerant(CMG30,CMG57,FH2,JMK10,CMG58,CMG59,CMG60,CMG61,CMG62).Plant height,spike length,spike diameter,single spike weight,and yield could serve as the objective evaluation indexes for assessing millet's drought tolerance during the mid to late growth stages,with the D value being suitable for drought evaluation.
foxtail milletdrought tolerant germplasm screeningmid to late growth stage drought toleranceevaluation methodInner Mongolia