Effects of returning different organic materials to soil on stoichiometric characteristics and extracellular enzyme activities of albic soil
To explore the effects of different organic materials returning on soil stoichiometric characteristics and extracellular enzyme activities in albic soil,a two-year positioning experiment was conducted in the albic soil region of Northeast China.Four treatments were established:conventional tillage control(CK,with no straw and organic fertilizer addition),straw returning(S),organic fertilizer returning(M)and straw combined with organic fertilizer returning(SM).The results indicated that,compared with the control,the application of organic materials increased soil organic carbon,total nitrogen,and total phosphorus contents increased by 1.81%~6.91%,1.54%~7.48%,and 2.04%~7.26%,respectively,but had no significant effect on soil C∶N,C∶P and N∶P ratios.Fur-thermore,the application of organic materials significantly increased the activities of soil carbon,nitrogen and phos-phorus acquisition enzymes,with the most pronounced increase observed in soil nitrogen acquisition enzyme activi-ty,ranging from 60.03%to 131.15%.Notably,the effect of organic fertilizer returning to the field and straw com-bined with organic fertilizer returning on the three soil acquisition enzymes was better than that of straw returning treatment.The results from the extracellular enzyme stoichiometric scatter plot showed that the soil microbial com-munity was jointly limited by carbon and phosphorus,and the input of organic materials could alleviate this limita-tion.Based on random forest analysis,it was discovered that soil available phosphorus,available nitrogen,microbi-al biomass carbon,microbial biomass nitrogen and microbial biomass phosphorus were important factors affecting soil microbial carbon and phosphorus limitation indicators.