Genome-wide identification of BnAR gene family and expression analysis under low temperature stress in Brassica napus L.
To understand the basic information,evolutionary relationship and the function of BnAR genes under cold stress in Brassica napus L.,the BnAR gene family of B.napus were identified in this study,and the ex-pression of family members under low temperature conditions were explored.The results showed that 14 BnAR genes were identified in B.napus.They were distributed on eight chromosomes and renamed as BnAR-1 to BnAR-14,di-viding into ADC and DapDC subgroups.Promoter cis-element analysis showed that the promoter region of BnAR genes contained various elements related to hormone and stress response.Intra-group collinearity analysis of B.napus showed that 11 pairs of genes had collinearity relationship,and Ka/Ks analysis showed that the ratio of all homolo-gous gene pairs were less than 0.5,indicating that the BnAR gene family was subjected to strict purification selec-tion in the evolutionary process.Transcriptome data analysis indicated that all 14 BnAR genes responded to low tem-perature stress,among which four genes,BnAR-4,BnAR-5,BnAR-11 and BnAR-13 were strongly subjected to low temperature stress.The results of qRT-PCR were highly consistent with the transcriptome data,so these four genes could be used as candidate genes for subsequent functional verification.
Brassica napus L.BnAR gene familybioinformaticsexpression patternlow temperature stress
万方数据
维普
