Identification and application of walnut rot biocontrol fungus Streptomyes deccanensis
To identify the biocontrol strains with high control effect on walnut rot and promote the germination of walnut seeds,soil was collected from the walnut orchard of a walnut forest farm in Wensu County,Xinjiang.Bio-control bacteria were isolated and screened by a dilution coating method.The biocontrol bacteria were identified by morphological characteristics,physiological and biochemical characteristics combined with molecular biology,and their antibacterial ability,disease prevention effect and promoting effect on walnut seed germination were studied.A strain of Streptomyces F-04(CGMCC No.29519)with broad-spectrum antibacterial activity was isolated,and it was identified as Streptomyes deccanensis.The inhibitory rate of this strain against Cytospora chrysosperma was 81.75％,and its fermentation filtrate achieved an 87.24％control effect on walnut branch rot.At a concentration of 150 mg·mL-1,the fermentation filtrate resulted in the highest germination rate of 85.32％,while the seed failure rate was the lowest at 9.32％.This demonstrates a significant promoting effect on walnut seed germination.
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