Analysis and comprehensive screening of genetic diversity of agronomic and quality traits of high generation millet lines
From 2022 to 2023,the agronomic traits and nutritional quality traits of 75 high-generation foxtail millet lines were determined,and genetic variation analysis was examined by genetic diversity analysis,fuzzy func-tion method,cluster analysis and principal component analysis.The results showed that the coefficient of variation of agronomic traits of millet was ear coarseness>yield>stem diameter>spike length>internodes under the pani-cle>weight per spike>leaf>leaf length>leaf width>plant height>growth period,with the coefficient of varia-tion ranged from 5.82%~16.07%,and the coefficient of variation of basic nutritional quality traits was fat content>protein content>fiber content>ash content>water content>carbohydrate content,with the coefficient of varia-tion ranged from 1.27%~19.13%.The genetic diversity index was the largest in grain weight(2.09),the smallest in plant height(0.62)and carbohydrate(0.47).The largest annual variation was in panicle diameter(30.01%),and the smallest variation was in carbohydrates(1.27%).When the square euclidean distance was 15,the tested varieties are divided into five groups.Category Ⅰ included 27 strains,which had a short growth period and could be used as breeding materials for early-maturing varieties.Category Ⅱ included 4 strains,which had high plant height and leaf length,and could be used as hay fodder breeding materials.Category Ⅲ included 16 strains,which had high spike length,single spike weight and yield,and could be used as high-yield breeding materials.Category Ⅳincluded 13 strains,which were short in plant height,thick stems and long internodes under panicles,which could be used as lodging resistance breeding materials.Category V included 15 strains,which had low water and fat con-tent,high carbohydrate,and protein content,and could be used as high-quality nutritional breeding materials.The contribution rates of the six principal components were 19.524%,16.943%,13.893%,9.057%,8.626%and 6.074%,respectively,and the cumulative contribution rate was 74.117%.According to the score of principal compo-nent factor and membership function,five lines with good comprehensive traits were screened out,which were 19(Y35)-18-4,19(Y35)-18-6,19(T2)-42-2,19(J-5)-45-2,and 19(Y34)-17-35.In this study,the ge-netic variation of agronomic and basic nutritional quality traits of high-generation millet lines was analyzed,and the breeding materials with excellent comprehensive traits were screened,providing a scientific basis for the breeding of new millet varieties in western Henan.