Soil ciliate community structure and relationship with environmental factors in sea buckthorn forest in Nianchu river basin
Sea buckthorn forest has important economic,ecological and social benefits,and plays an important role in the ecological and environmental security of the Qinghai-Tibet Plateau.Soil ciliates can be used as indicators of the dynamic changes of sea buckthorn forest ecosystem.In May(spring),August(summer)and October(autumn)of 2020,five typical natural sea buckthorn forests in the Nianchu river basin were selected as sample sites according to the distribution characteristics of sea buckthorn forests,and a total of 75 soil samples were obtained.Culture and identification of soil ciliates were performed by"non-submerged petri dish method"and"in vivo observation method",and protein silver staining was used to further confirm the species that were difficult to identify.A total of 90 species of soil ciliates were identified in this study,belonging to 47 genera,30 families,20 orders,and 10 classes.The dominant group was Sporadotrichia(15 species),accounting for 16.67％of the total species.The results of one-way ANOVA showed that the diversity index of soil ciliate in sea buckthorn forest was different between seasons(P＜0.05).The cooccurrence network showed that the soil ciliate species interaction in sea buckthorn forest was more complex in spring.There were 28 pairs of dominant species with high niche overlap(Oik＞0.6),accounting for 77.78％of the total logarithm,indicating fierce competition among dominant species.Mantel analysis showed that pH and available potassium were the main factors affecting soil ciliate diversity index in sea buckthorn forest.Redundancy analysis(RDA)showed that soil water content,available phosphorus,total nitrogen and available potassium were the environmental factors affecting the distribution of ciliate species in the soil of sea buckthorn forest in the Nianchu river basin.The results of this study are helpful to understand the changes of sea buckthorn forest ecosystem in the Nianchu river basin,and provide basic data support for ecological and environmental protection and sustainable development of the Qinghai-Tibet Plateau.
The Nianchu river basinsea buckthorn forestsoil ciliatecommunity structure
NETL
NSTL
万方数据
