汾河平原小麦成熟前期根际土丛枝菌根真菌群落结构的空间变异
Spatial variation of community structure of arbuscular mycorrhizal fungi in wheat rhizosphere soil in Fenhe Plain
郭海涛 1曹艳峰 1云秋皓1
作者信息
- 1. 山西师范大学地理科学学院,太原 030031
- 折叠
摘要
为揭示汾河平原小麦根际土丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)群落结构的空间变异,研究选取了汾河平原的14个麦田样点,采用Illumina测序平台检测了小麦根际土 AMF的群落结构.结果表明:14个样点均有AMF的3科6属,其中球囊霉科(Glomeraceae)和近明球囊霉科(Claroideoglomeraceae)为优势科,球囊霉科中别的属类(Glomeraceae;other)和近明球囊霉属(Claroideoglomus)为优势属;AMF各科属的相对丰富度从南向北沿纬度增加没有明显的变化规律;AMF群落的不相似性与地理距离不显著相关(P＞0.05),而与土壤环境、气候环境的不相似性显著相关(P＜0.05);土壤环境对AMF群落变异的单独贡献率最高,为34.5％,而气候环境、地理距离的单独贡献率均很低,分别为3.6％、0.4％;冗余分析显示,AMF群落变异主要与土壤变量如N/P、pH、Clay、AP显著相关(P＜0.05),其中N/P对AMF群落变异的影响最大(解释率为23.4％).研究表明,在纬度跨度较小的局域尺度上,AMF群落结构的空间变异主要被土壤环境变量驱动,而不是地理距离.
Abstract
The spatial pattern of farmland soil microorganisms is one of the important research contents in soil microbial biogeography.Arbuscular mycorrhizal fungi are one of the important fungi flora that can form a symbiotic associate with plant roots.In this study,the 14 wheat farmland sites along latitude increasing from south to north in the Fenhe Plain were selected,three plots(1 m x 1m)were set in each site,and soil samples in each plot were collected by 5-point sampling method.Soil samples from the five sampling points were mixed to obtain one composited sample.Illumina sequencing was adopted to predict the spatial variations of arbuscular mycorrhizal fungi community composition.Meanwhile,soil physical and chemical properties were also measured to reveal their relationships with arbuscular mycorrhizal fungi community.The three families and six genera of arbuscular mycorrhizal fungi were observed from all sequences in the 14 sites.Glomeraceae and Claroideoglomeraceae were the dominant families.The other genera of Glomeraceae and Claroideoglomus were the dominant genera.Each flora at family and genus of arbuscular mycorrhizal fungi had no obvious regularity along latitude increasing from south to north.The dissimilarity of arbuscular mycorrhizal fungi community was not significantly related to geographical distance(P＞0.05),but was significantly related to environmental dissimilarity(P＜0.05).The contribution rate of soil environment to the variation of arbuscular mycorrhizal fungi community was the highest(34.5％).By contrast,the contribution rate of climate environment(3.6％)and geographical distance(0.4％)was very low.The results of redundancy analysis(RD A)showed that the variation of arbuscular mycorrhizal fungi community was mainly correlated with soil environmental variables such as N/P,pH,Clay and AP(all P＜0.05).Especially,N/P had the greatest effect,and its explaining rate was 23.4％.These results suggest that the spatial variation of arbuscular mycorrhizal fungi community structure is mainly driven by soil environmental variables rather than geographical distance at local scales with a smaller latitude span.These findings can provide some references for the excavation of soil microbial resources and their potential functions in the farmland ecology.
关键词
根际土壤/丛枝菌根真菌/群落结构/空间变异Key words
rhizosphere soil/arbuscular mycorrhizal fungi/community structure/spatial variation引用本文复制引用
基金项目
山西省自然科学基金(20210302123331)
出版年
2024
国家科技期刊平台
NSTL
万方数据