Spatial variation of community structure of arbuscular mycorrhizal fungi in wheat rhizosphere soil in Fenhe Plain
The spatial pattern of farmland soil microorganisms is one of the important research contents in soil microbial biogeography.Arbuscular mycorrhizal fungi are one of the important fungi flora that can form a symbiotic associate with plant roots.In this study,the 14 wheat farmland sites along latitude increasing from south to north in the Fenhe Plain were selected,three plots(1 m x 1m)were set in each site,and soil samples in each plot were collected by 5-point sampling method.Soil samples from the five sampling points were mixed to obtain one composited sample.Illumina sequencing was adopted to predict the spatial variations of arbuscular mycorrhizal fungi community composition.Meanwhile,soil physical and chemical properties were also measured to reveal their relationships with arbuscular mycorrhizal fungi community.The three families and six genera of arbuscular mycorrhizal fungi were observed from all sequences in the 14 sites.Glomeraceae and Claroideoglomeraceae were the dominant families.The other genera of Glomeraceae and Claroideoglomus were the dominant genera.Each flora at family and genus of arbuscular mycorrhizal fungi had no obvious regularity along latitude increasing from south to north.The dissimilarity of arbuscular mycorrhizal fungi community was not significantly related to geographical distance(P＞0.05),but was significantly related to environmental dissimilarity(P＜0.05).The contribution rate of soil environment to the variation of arbuscular mycorrhizal fungi community was the highest(34.5％).By contrast,the contribution rate of climate environment(3.6％)and geographical distance(0.4％)was very low.The results of redundancy analysis(RD A)showed that the variation of arbuscular mycorrhizal fungi community was mainly correlated with soil environmental variables such as N/P,pH,Clay and AP(all P＜0.05).Especially,N/P had the greatest effect,and its explaining rate was 23.4％.These results suggest that the spatial variation of arbuscular mycorrhizal fungi community structure is mainly driven by soil environmental variables rather than geographical distance at local scales with a smaller latitude span.These findings can provide some references for the excavation of soil microbial resources and their potential functions in the farmland ecology.
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