Vertical distribution of microbial communities in saline-alkali soil of the arid area of middle Gansu
The typical crust saline soil(HY),desert saline soil(XQ),and oasis farmland saline alkali soil(YC)were sampled from the arid area of middle Gansu to analyze soluble salt ion content,bacterial and fungal diversity,and community structure along vertical profile[top soil A(0-20cm),core soil B(20-40cm)and bottom soil horizon E(40-80cm)].This work is conducted to explore the relationship between distribution of microbial community and water and salt contents along vertical depth.The results indicate that the sampled soils belong to severe sulfate type saline alkali soil,salt ion are richer in A horizon,and the degree of salinization is ranked as HY＞YC＞XQ.The results of 16S rDNA and ITS amplicon high-throughput sequencing indicate that the Actinobacteriota,Proteobacteria,Chloroflexi,Firmicutes,Ascomycota,Basidiomycota are all dominated in throughout profiles.The bacterial diversity appears linear descent,"U"and unimodal patterns along depths.Fungal diversity presents as linear descent,and shows a more significant decrease than bacteria.Distribution of microbial community are associated with soil heterogeneity in profile after long-term water-salt transport.Among them,soil moisture content is a determining factor,which shaped microbial community structure jointly with total salt and pH.These results contribute to understanding the mechanisms of microbial community for soil salinization,and have practical guiding significance for the sustainable utilization and ecologically restoration for saline alkali soil in this region.
saline-alkali soilmicrobial diversitycommunity compositionvertical distributionarid area
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