Effects of slurry addition on soil enzyme activity and microorganisms in the rhizosphere of Medicago sativa L.
The unique structure and composition of slurry confer positive effects on water retention,fertilization,and soil habitat construction.This provides an important theoretical basis and broad application prospects for the ecological restoration of degraded land and utilization in agriculture,forestry,and animal husbandry.Clearly understanding the pros and cons of slurry on plants,microorganisms,and their soil biological environment is a prerequisite for its ecological utilization.To explore the biological environmental characteristics of slurry and seek new directions for its resource utilization,as well as to improve its utilization efficiency,this study designed four mixing ratios of slurry and sand(0:4 for CK,2:2 for CS-1,3:1 for CS-2,and 4:0 for CS-3)for a pot planting experiment in Medicago sativa L.The effects of slurry on soil enzyme activity and soil microbial community structure in the rhizosphere of alfalfa were analyzed.The results showed that:1)Soil urease and hydrogen peroxide enzyme activities decreased with the increase of slurry addition.Dehydrogenase activity increased with the increase of slurry addition,with the highest alkaline phosphatase activity observed in CS-1 soil.2)Soil bacterial diversity index decreased with the increase of slurry addition,while the highest soil fungal diversity index was observed in CS-1 treatment.3)Proteobacteria were the dominant bacterial phylum in all treatments,while Ascomycota was the dominant fungal phylum.NMDS analysis showed that slurry had a significant impact on bacterial community structure.4)Correlation analysis revealed significant correlations(P＜0.05)between soil urease activity,hydrogen peroxide enzyme activity,dehydrogenase activity,and bacterial diversity index,while soil enzyme activity was not significantly correlated with fungal community(P＞0.05).Redundancy analysis showed significant correlations(P＜0.05)between multiple dominant microbial populations and soil enzyme activity.In summary,slurry addition increased the abundance and diversity of fungal species in the rhizosphere of alfalfa while reducing bacterial abundance and diversity,leading to specific differentiation of bacterial populations.CS-1 treatment showed relatively higher soil microbial diversity and enzyme activity,providing a reference for the resource utilization of slurry.
万方数据
维普
