Identification,biological characteristics and cultivation of a wild Armillaria mellea
In this study,a wild macrofungus was isolated from dead Caragana branches.Morphological characteristics and molecular phylogenetic analysis were used to identify the strain.Single factor and orthogonal experiments were applied to study the biological characteristics of the fungus,the culture medium screening and the semi-wild domestication culture.The fungus was identified as Armillaria mellea.For the growth of A.mellea,the optimum carbon source was fructose,nitrogen source was yeast extract,temperature was 20℃,pH was 6.0.The optimal medium formula for A.mellea was as follows:fructose 20g,yeast extract 2g,pH 5.0,temperature 20℃,AGAR 20g,water 1L.The optimal culture medium for the expansion of the strains was buckwheat 98%,white sugar 1%,gypsum 1%.The mushroom was grown in semi-imitation wild cultivation mode.The flat stubble branches of Caragana were buried in the field under natural conditions combined with artificial management.The fruiting bodies grew at the end of September of the following year.The success of this experiment offers valuable insights for the artificial cultivation of wild A.mellea and the further exploration and development of the utilization of Caragana resources.
utilization of Caragana resourcesArmillaria melleabiological characteristicscultivation
万方数据
维普
