Analysis of oral microbial diversity in patients with erectile dysfunction by 16S rRNA gene sequencing
Objective To analyze the differences in the structure of oral microbiota between patients with erectile dysfunction(ED)and healthy controls by 16S rRNA high-throughput sequencing,and to explore the relationship between ED and oral microbiota.Methods Fifteen ED patients who were 20-65 years old and treated at Department of Urological Surgery,First Hospital,Guizhou University of Traditional Chinese Medicine(ED group)and 15 healthy controls(normal control group)were selected.Their saliva samples were collected for the 16S rRNA high-throughput sequencing.The structures of the bacterial flora in the saliva samples of both groups were compared and analyzed.The independent-sample t test,non-parameter test,and x2 test were applied.Results The compositions of core dominant bacteria in the salivary microbiota of the ED group and the normal control group were similar;the top ten dominant bacteria at the genus level were Fusobacterium,Reil,Neisseria,Haemophilus,Prevotella,Veillonella,Isovalinus,Streptococcus,Porphyromonas,and Actinobacteria.There were statistical differences in the abundances of different flora in the saliva samples between the two groups(all P＜0.05).The abundance of Streptococcus in the ED group was significantly higher than that in the normal control group[8.59(4.13,11.75)％vs.4.23(3.25,7.48)％],while the abundance of Fusobacterium in the ED group was significantly lower than that in the normal control group[9.38(5.61,14.91)％vs.14.13(7.92,19.03)％],with statistical differences between the two groups(both P＜0.05).Conclusions The oral microbiota of ED patients and healthy people have the same core composition skeleton,but there are some differences in the microbiota structure,which may be closely related to the occurrence and development of ED.However,the causal relationship and its related mechanisms should be further explored.
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