Correlation of HPV expression with TAP,CD8,and HLA in laryngeal squamous cell carcinoma and clinical significance
Objective To detect the expression levels of P16,TAP1,TAP2,HLA,and CD8 in laryngeal squamous cell carcinoma,and to explore the expression levels of TAP1,TAP2,HLA,and CD8 in laryngeal squamous cell carcinoma,and their correlations with HPV infection and the clinical significance.Methods The tissue wax blocks from 84 patients with laryngeal squamous cell carcinoma surgically removed from Binzhou Medical University Hospital from January 2013 to December 2018 were selected as the study objects,including 68 males and 16 females;they were 45-78 years old,with a median age of 63.The expression levels of P16,TAP1,TAP2,HLA,and CD8 in the 84 cases of laryngeal squamous cell carcinoma was detected by the immunohistochemical EnVision method.The relationship between P16 and the clinicopathological features was analyzed.x2 test,Fisher exact test,and Spearman rank correlation test were used to analyze the correlation between HPV infection and the above protein expression levels.The Kaplan-Meier survival analysis was performed,and the Cox proportional risk regression model was used for the multivariate survival analysis.Results The positive expression rate of P16 in laryngeal squamous cell carcinoma was 11.9％(10/84),while the expression was negative in the adjacent tissue.The positive rate of CD8 in laryngeal squamous cell carcinoma was 38.1％(32/84),which was lower than that in the adjacent tissue[56％(47/84)].The positive rates of TAP1,TAP2,and HLA in the laryngeal squamous cell carcinoma tissue were 41.7％(35/84),29.8％(25/84),and 42.8％(36/84),respectively,which were lower than those in the adjacent tissue[69％(58/84),56％(47/84),and 57.1％(48/84)];there was a statistical difference in the expression of TAP2 between the cancer and adjacent tissues(x2=5.80,P＜0.05).The expression of CD8 in the laryngeal squamous cell carcinoma tissue was correlated with clinical stage,and the difference was statistically significant(x2=6.57,P＜0.05).The expressions of TAP1,TAP2,HLA,and P16 were not correlated with the clinicopathological factors.The Spearman correlation analysis showed that the TAP1 expression was positively correlated with TAP2 and CD8(r=0.295 and 0.232,both P＜0.05),and the HLA expression was positively correlated with the CD8 expression(r=0.232,P＜0.05).Conclusion The low expression levels of TAP1,TAP2,HLA,and CD8 in laryngeal squamous cell carcinoma tissue suggests that the tumor may down-regulate TAP1 and TAP2,resulting in abnormal HLA expression,thereby inhibits the immune response of CD8 and the immune response of the body,providing theoretical support for the mechanism of tumor immune escape.
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