Intestinal flora imbalance in patients with polycystic ovary syndrome and its relationship with insulin resistance
Objective To investigate the intestinal flora imbalance in patients with polycystic ovary syndrome(PCOS)and its relationship with insulin resistance.Methods Fifty(25.98±0.95)years old patients with PCOS treated at Shiyan People's Hospital from June 2020 to June 2022 were selected as a study group.Fifty(26.02±1.01)years old healthy women with regular menstruation during the same period were selected as a healthy control group.All the subjects were tested for insulin resistance.The characteristics of intestinal flora distribution were analyzed.The intestinal flora distribution characteristics and insulin indicators of the two groups were compared to analyze the intestinal flora imbalance and its correlation with insulin resistance in the patients with PCOS.t test and x2 test were used for the statistical analysis.Results After the cluster analysis,372 operational taxonomic unit(OTU)representative sequences were obtained,including 60 OTU sequences specific to the study group and 90 OTU sequences specific to the healthy control group;there were 111 shared OTU sequences in the two groups;the results of Alpha analysis showed that the number of OTU and Shannon index in the study group were lower than those in the healthy control group(both P＜0.05).The relative abundance of Firmicutes at the phylum level in the study group was higher than that in the healthy control group,and the relative abundance of Bacteroidetes in the study group was lower than that in the healthy control group(both P＜0.05);the relative abundances of Brucella,Bacteroidetes,and Faecobacter at the genus level were lower than those in the healthy control group,and the relative abundance of Eubacterium Hoveri in the study group was higher than that in the control group(all P＜0.05).The fasting insulin(FINS)and insulin resistance index(HOMA-IR)in the study group were higher than those in the healthy control group[(9.55± 1.98)mIU/L vs.(6.71±1.92)mIU/L and(2.08±0.50)vs.(1.49±0.40);both P＜0.05].Bacteroides was positively correlated with HOMA-IR(r=0.281,P＜0.05),and fecal bacillus negatively with HOMA-IR(r=-0.331,P＜0.05).Conclusion Patients with PCOS have microflora dysregulation,and the occurrence of intestinal microflora dysregulation is closely related to insulin resistance.The detection of their intestinal microflora level can provide references and basis for the clinical diagnosis and treatment of patients with PCOS.
万方数据
维普
