Study on the infection status of pediatric bacterial diarrhea and application of microbial testing techniques
Objective To analyze the infection status of bacterial diarrhea in children and the application effects of different microbial testing techniques.Methods This was a retrospective study.The cases included were 100 children with bacterial diarrhea admitted to Ruyang County Hospital of Traditional Chinese Medicine from January 2021 to April 2023,and 25 healthy volunteers were used as controls.There were 52 boys and 48 girls in the case group,aged 1-10(5.53±1.46)years.There were 13 boys and 12 girls in the control group,aged 2-9(5.44±1.28)years.The pathogenic microorganism infection of the cases was counted according to the results of pathogenic microbiological culture.Using the results of pathogenic microbiological culture as the gold standard,the diagnostic efficacies of latex agglutination test(LA)and enzyme-linked immunosorbent assay(ELISA)for bacterial diarrhea in children were compared.x2test and independent sample t test were used.Results According to statistics,bacterial diarrhea was more common in children aged 2-6 years,mostly occurring from October to February,boys more than girls.Among the 100 children with bacterial diarrhea,the infection rate of Shigella was 36.00％(36/100),that of pathogenic Escherichia coli was 25.00％(25/100),that of toxigenic Escherichia coli was 20.00％(20/100),that of fetal Campylobacter was 10.00％(10/100),and that of Salmonella was 9.00％(9/100).The diagnostic accuracy,sensitivity,and specificity of LA detection for pediatric bacterial diarrhea were 87.20％(109/125),88.00％(88/100),and 84.00％(21/25),respectively,which were all higher than those of ELISA[71.20％(89/125),72.00％(72/100),and 68.00％(17/25)],with statistically significant differences(all P＜0.05).Conclusions Bacterial diarrhea is more common in autumn and winter seasons,and it is more common in children aged 2-6 years.Shigella is a common type of infection.LA can achieve effective diagnosis of bacterial diarrhea in children,and its test results may serve as an important basis for guiding clinical treatment.
万方数据
维普
