Effect of PTBP3 on proliferation and migration of esophageal squamous cell carcinoma and its mechanism
Objective To explore the role and potential mechanism of polypyrimidine tract binding protein 3(PTBP3)in esophageal squamous cell carcinoma.Methods Six specimens of esophageal squamous cell carcinoma and six specimens of its adjacent normal tissue were collected from Jining First People's Hospital from January 2021 to May 2023.The expressions of PTBP3 in the tissue and the cells were determined by real-time quantitative polymerase chain reaction(qRT-PCR)and Western blotting;the CCK8 assay kit was used to determine the ESCC cells'viability;the scratch experiment was used to determine the ESCC cells'migration ability.The UALCAN and TCGA databases were searched to analyze the expression difference of PTBP3 between the esophageal cancer tissue and the normal esophageal tissue,and the relationship between PTBP3 and miR29 family.t test was used.Results The expression of PTBP3 in the ESCC tissue was higher than that in the normal esophageal tissue,with a statistical difference(P＜0.05).The results of qRT-PCR showed that the expressions of miR-29a-3p and miR-29c-3p in the PTBP3 knocked down group were lower than those in the control virus group in the 3 ESCC cells(KYSE30,ECA109,and KYSE150)(t=29.007,7.057,4.844,12.625,15.612,and 18.861;all P＜0.05).In the ESCC tissue,the expressions of miR-29a-3p and miR-29c-3p were correlated with the expression of PTBP3.Conclusion PTBP3 may be an important molecular marker and potential therapeutic target for ESCC.It can provide new ideas and a method for the early diagnosis and a treatment of ESCC.
万方数据
维普
