Expression of CLCA4 in non-small cell lung cancer tissue and its correlations with pathology and prognosis
Objective To investigate the expression of calcium-activated chloride channel regulator 4(CLCA4)in non-small cell lung cancer(NSCLC)tumor tissues and its correlations with pathology and prognosis.Methods A total of 106 patients with NSCLC admitted to Shangluo Central Hospital from April 2019 to April 2022 were prospectively selected for the study,including 64 males and 42 females,aged(56.53±7.54)years.All the patients underwent radical resection of NSCLC.The positive expression of CLCA4 in cancer tissues and adjacent normal tissues was detected by immunohistochemistry,and the relative expression of CLCA4 mRNA in cancer tissues and adjacent normal tissues was detected by real-time fluorescent quantitative reverse transcriptase-polymerase chain reaction(qRT-PCR).The patients were divided into a progression-free survival(DFS)group and a non-DFS group according to the 1-year follow-up outcomes.t test and x2 test were used for statistical analysis.Multivariate logistic stepwise regression model,Kaplan-Meier survival curve analysis,and log-rank test were used to analyze the patients'prognostic factors.The receiver operating characteristic curve(ROC)was drawn to analyze the relationships between CLCA4 and pathology and prognosis.Results The positive rate of CLCA4[45.28％(48/106)]and the relative expression level of CLCA4 mRNA[(0.61±0.14)]in cancer tissues were lower than those in adjacent normal tissues[66.98％(71/106)and(1.07±0.23)](x2=10.134,P＜0.001;t=17.589,P＜0.001).The expression level of CLCA4 mRNA in stage Ⅱ patients was higher than that in stage Ⅲ A patients[(0.69±0.15)vs.(0.61±0.13)](t=2.914,P=0.004).The proportion of stage Ⅲ A in the non-DFS group was higher than that in the DFS group[82.76％(48/58)vs.33.33％(16/48)](x2=26.819,P＜0.001),and the expression of CLCA4 mRNA was lower than that in the DFS group[(0.54±0.13)vs.(0.69±0.17)](t=5.514,P＜0.001).TNM stage(OR=4.031,95％CI 1.429-11.364,P=0.003)and CLCA4 mRNA expression level(OR=0.310,95％CI 0.109-0.873,P=0.003)were the influencing factors for DFS in NSCLC patients.The sensitivity of CLCA4 mRNA expression in predicting DFS in NSCLC patients was 0.802(95％CI 0.715-0.893),the specificity was 0.809(95％CI 0.721-0.904),and the area under the curve(AUC)was 0.857(95％CI 0.782-0.935).The survival curves of the patients in different CLCA4 mRNA expression groups were significantly different(Log-rank x2=6.006,P=0.014).Conclusion The expression of CLCA4 in cancer tissue of NSCLC patients is suppressed,which is related to pathological progression as well as prognosis.
万方数据
维普
