Long non-coding RNA IGF2BP2-AS1 regulates the migration and proliferation of laryngeal cancer cells through miR-375
Objective To investigate the effect of long non-coding RNA(lncRNA)insulin-like growth factor 2 mRNA binding protein 2(IGF2BP2)-AS1 on the migration and proliferation of laryngeal cancer cells by regulating the expression of microRNA-375(miR-375).Methods The research period was from January 2022 to November 2023,the research place was the central laboratory of Luoyang Central Hospital Affiliated to Zhengzhou University,and the research type was basic research.qPCR was used to detect the expression of IGF2BP2-AS1 in laryngeal cancer cell lines AMC-NH-8,TU159,TU138,and TU686 and bronchial epithelial cell line 16HBE.Taking TU686 cells as the research object,the TU686 cells were divided into a si-NC group and a si-IGF2BP2-AS1 group,and IGF2BP2-AS1 small interfering RNA was used to down-regulate the expression level of IGF2BP2-AS1.The scratch healing assay and CCK-8 method were used to analyze the effects of knocking down IGF2BP2-AS1 on the migration and proliferation of TU686 cells.Dual luciferase reporter gene experiment verified the targeting relationship between IGF2BP2-AS1 and miR-375.qPCR was used to detect the effect of knocking down IGF2BP2-AS1 on the expression of miR-375.Western blotting was used to detect the expressions of migration proteins(N-Cadherin and FOXC2)and proliferation proteins(CDK2 and Cyclin A)in cells.One-way ANOVA and t test were used.Results Compared with 16HBE cells,IGF2BP2-AS1 was highly expressed in laryngeal cancer cell lines(F=37.42,P＜0.01).The scratch healing rate of TU686 cells in the si-IGF2BP2-AS1 group was lower than that in the si-NC group[(28.16±6.13)％vs.(65.08±3.82)％],with a statistically significant difference(t=5.11,P＜0.01).The proliferation capacity of TU686 cells in the si-IGF2BP2-AS1 group was lower than that in the si-NC group 2,3,4,and 5 days after planking(t=3.83,3.17,3.02,and 6.31,all P＜0.01).In IGF2BP2-AS1-Wt,the relative luciferase activity of the miR-375 group was lower than that of the miR-NC group(t=8.95,P＜0.01).The expression of miR-375 in TU686 cells in the si-IGF2BP2-AS1 group was higher than that in the si-NC group[(4.95±0.49)vs.(1.02±0.40)],with a statistically significant difference(t=6.23,P＜0.01).Compared with the si-NC group,the expression levels of migration proteins(N-Cadherin and FOXC2)and proliferation proteins(CDK2 and Cyclin A)were reduced in TU686 cells after knocking down IGF2BP2-AS1.Conclusion IGF2BP2-AS1 is highly expressed in laryngeal cancer cell lines,and knocking down IGF2BP2-AS1 inhibits the migration and proliferation of laryngeal cancer TU686 cells by regulating the expression of miR-375.
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