Objective To investigate the clinical value of sandwich cup centrifuge smear method for detecting acid-fast bacteria.Methods The test results of sputum and bronchial lavage fluid by smear staining and mycobacterium isolation were collated from the LIS system of the Department of Clinical Laboratory of Guangzhou Chest Hospital from January to July 2021.The results of direct smear method and sandwich cup centrifuge smear method for acid-fast bacteria of multiple sputum and bronchial lavage fluid samples which were collected within one week interval from the same patient were analyzed retrospectively.Results Of the 241 sputum samples,178 bronchial lavage fluid samples,and all 419 samples tested,the positive rates of direct smear method and sandwich cup centrifuge smear method were 15.35％(37/241)and 13.69％(33/241),15.17％(27/178)and 12.92％(23/178),15.27％(64/419)and 13.37％(56/419),respectively.Of the 65 positive sputum samples,50 positive bronchial lavage fluid samples,and all 115 positive samples detected by mycobacterium isolation during the same period,the positive rates of direct smear method and sandwich cup centrifuge smear method were 56.52％(65/115)and 50.78％(65/128),54.34％(50/92)and 45.88％(50/109),55.56％(115/207)and 48.73％(115/236),respectively.In the 241 sputum samples,the proportions of samples with direct smear(+)and sandwich cup centrifuge smear(+),direct smear(+)and sandwich cup centrifuge smear(-),direct smear(-)and sandwich cup centrifuge smear(+),and direct smear(-)and sandwich cup centrifuge smear(-)were 12.03％(29/241),3.32％(8/241),1.66％(4/241),and 82.99％(200/241),respectively,and those in the 178 bronchial lavage fluid samples were 8.99％(16/178),6.18％(11/178),3.93％(7/178),and 80.90％(144/178),respectively.Conclusion The overall positive rate of sandwich cup centrifuge smear method is slightly lower than that of direct smear method,but it has positive clinical application value because it can find a considerable proportion of etiologically positive tuberculosis patients that can not be found by direct smear method,especially in bronchial lavage fluid samples.
Acid-fast bacteriaAcid-fast stainingSandwich cup centrifuge smear methodDirect smear method
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