Objective To establish the quality evaluation method of fresh fruit of Solanum nigrum Linn.formula granules based on the quality indexes of standard decoction.Methods Ten batches of standard decoction and 3 batches of formula granules of fresh fruit of Solanum nigrum Linn.were prepared and the paste rate was calculated.Thin layer chromatography(TLC)was used to identify the specific properties of the formula granules.The contents of solasonine and solamargine in the formula granules were determined by ultra-high performance liquid chromatography(HPLC).The fingerprint of formula granules was established,and 4 characteristic peaks and their relative retention time were determined.Results The paste rate of standard decoction was(18.21±1.87)％,the paste rate of formula granules ranged from 12.75％to 23.67％,and the paste rates of the three batches of formula granules were 15.90％,21.26％,and 18.01％,respectively.In the standard decoction,the content of solasonine was(24.54±1.24)mg·g-1,the content of solamargine was(31.34±1.65)mg·g-1.In the three batches of formula granules,the contents of solasonine were 19.3 mg·g-1,18.5 mg·g-1,and 20.4 mg·g-1,and the contents of solamargine were 23.9 mg·g-1,22.9 mg·g-1,and 25.8 mg·g-1.Four common characteristic peaks were identified,and the relative retention time values of the other three peaks were 1.13,1.70,and 1.85 with solasonine as the reference peak.Conclusion This study proved that the quality of formula granules of fresh fruit of Solanum nigrum Linn.was consistent with standard decoction,and provided data support for the formulation of quality standards for the formula granules.
Quality controlFresh fruit of Solanum nigrum Linn.Standard decoctionFormula granules
国家科技期刊平台
NSTL
万方数据
