Effects of HSYA on biological characteristics of human lens epithelial cell line SRA01/04
Objective To explore the effects of hydroxysafflor yellow A(HSYA)on the proliferation,migration,and epithelial-mesenchymal transition(EMT)of human lens epithelial cell line SRA01/04(HLEC-SRA01/04)induced by recombinant human epidermal growth factor(rhEGF).Methods This study was from June 2022 to September 2023.The HLEC-SRA01/04 induced by rhEGF were treated with different concentrations of HSYA solution for different durations.The proliferation status of the cells of each group was detected by the MTT assay,and the IC50 value was calculated.The cell scratch test and Transwell chamber were used to measure the migration ability of the cells in different concentration groups.Reverse transcription quantitative polymerase chain reaction(RT-qPCR)and Western Blot were adopted to determine the mRNA and protein expression levels of proliferating cell nuclear antigen(PCNA)and vimentin involved in EMT in the cells of different concentration groups.The measurement data were compared by the t test.Results The MTT assay results showed that the proliferation inhibition rates were(12.1±0.6)％,(19.0±3.8)％,(31.5±2.2)％,(53.7±0.4)％,and(70.8±0.3)％,respectively,and the IC50 value was(76.520±0.954)μmol/L after treating HLECs induced by 5 μg/L rhEGF with the HSYA solution of 20,40,60,80,and 100 μmol/L for 24 h;the proliferation inhibition rates were(14.3±3.7)％,(27.4±3.1)％,(42.6±2.7)％,(59.2±2.2)％,and(81.0±1.0)％,respectively,and the IC50 value was(66.094±2.508)μmol/L after treating HLECs induced by 5 μg/L rhEGF with the HSYA solution of 20,40,60,80,and 100 μmol/L for 48 h,indicating significant dose-dependent effects.The cell scratch test results showed that the 24 h cell migration rates of the blank group and the 0,20,40,70,and 100 μmol/L groups were(46.9±1.8)％,(90.0±1.5)％,(88.4±2.1)％,(43.3±6.6)％,(31.5±16.2)％,and(5.82±5.2)％,and the 48 h cell migration rates were(81.1±2.3)％,100％,(95.5±0.1)％,(72.6±3.5)％,(58.5±6.1)％,and(37.4±7.1)％,respectively.The Transwell chamber results showed that the numbers of cells in the blank group and the 0,20,40,70,and 100 μmol/L groups were(171.667±20.407),(290.222±24.135),(198.667±16.826),(161.222±5.981),(134.111±6.850),and(67.444±7.351),respectively;HSYA inhibited the migration of HLECs induced by rhEGF in a concentration-dependent manner.The RT-qPCR and Western Blot results proved that HSYA significantly down-regulated the mRNA and protein expressions of PCNA and Vimentin in HLECs induced by rhEGF in a dose-dependent manner.Conclusion HSYA remarkably inhibits the proliferation,migration,and EMT of HLEC-SRA01/04 induced by rhEGF,serving as a promising drug for preventing posterior capsular opacification.
万方数据
维普
