Expression levels of serum miR-16-5p and miR-155-5p and their relationships with prognosis in patients with infectious shock
Objective This study aimed to assess the serum levels of miR-16-5p and miR-155-5p in patients with infectious shock,and to explore their relationships with prognosis.Methods From June 2021 to July 2023,a total of 86 patients diagnosed with infectious shock were selected from the Emergency Department of Chang'an Hospital.There were 47 males and 39 females,45 cases＜60 years old and 41 cases ≥60 years old.These patients were categorized into three groups based on the severity of their condition:mild(30 patients),moderate(29 patients),and severe degree(27 patients).Over a one-month period,the survival status of these patients was monitored,leading to the formation of two groups:a death group(20 patients)and a survival group(66 patients).Comparative analyses of clinical data were conducted across each group.The correlations between the expression levels of miR-16-5p and miR-155-5p and the Acute Physiology and Chronic Health Evaluation(APACHE Ⅱ)score were analyzed.The receiver operating characteristic curve(ROC)was constructed to assess the prognostic diagnostic values of serum miR-16-5p and miR-155-5p in infectious shock.The risk factors of poor prognosis in patients with infectious shock were analyzed by logistic regression analysis.Independent sample t test,F test,x2 test,and Pearson correlation analysis were used.Results The heart rate(HR),C-reactive protein(CRP),APACHE Ⅱ score,and expression levels of miR-16-5p and miR-155-5p in the severe group were higher than those in the mild and moderate groups(all P＜0.05),but the mean arterial pressure(MAP),central venous pressure(CVP),and oxygenation index(OI)were lower than those in the mild and moderate groups(all P＜0.05).The CRP,APACHE Ⅱ score,and expression levels of miR-16-5p and miR-155-5p in the death group were higher than those in the survival group(all P＜0.05),but the OI was lower than that in the survival group(P＜0.05).Pearson correlation analysis showed that the expression levels of miR-16-5p and miR-155-5p were positively correlated with APACHE Ⅱ score(r=0.509 and 0.722,both P＜0.001).The area under the curve(AUC)of serum miR-16-5p combined with miR-155-5p in predicting poor prognosis in patients with infectious shock was 0.944,the 95％confidence interval(CI)was 0.894-0.994,the sensitivity was 90.0％,and the specificity was 84.8％,indicating high predictive efficacy.Both miR-16-5p and miR-155-5p were risk factors for poor prognosis in patients with infectious shock[OR=10.608(2.275-49.461)and 25.410(4.573-141.176),both P＜0.05].Conclusion In patients with infectious shock,serum expression levels of miR-16-5p and miR-155-5p are significantly associated with the condition's severity and prognosis.Thus,these two miRNAs may serve as biomarkers for predicting poor prognosis in such patients.
万方数据
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