摘要
目的 本研究旨在通过高效液相色谱法(HPLC)建立复方金菊口服液的指纹图谱,为其质量控制和评价提供科学依据.方法 采用Discovery C18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1％磷酸溶液梯度洗脱,流速为1.0 ml/min,柱温为30 ℃,检测波长245 nm.研究时间为2022年1月至2023年12月.结果 建立复方金菊口服液的指纹图谱,共标定21个共有峰,并指认其中6个共有峰.结论 HPLC简单、稳定,各峰分离良好,为复方金菊口服液的质量控制和评价提供科学依据.
Abstract
Objective To provide some scientific references for controlling the quality and evaluation of compound Jinju oral liquid establishing the fingerprint of compound Jinju oral liquid by high performance liquid chromatography(HPLC).Methods The Discovery C18 chromatography column(250.0 mm×4.6 mm,5 μm)was used;the mobile phase was acetonitrile-0.1％phosphate solution gradient ution;the flow rate was 1.0 ml/min;the column temperature was 30 ℃;the detection wavelength was 245 nm.The study was from January 2022 to December 2023.Results A fingerprint of compound Jinju oral solution was established.A total of 21 common peaks were calibrated,and 6 of the common peaks were identified.Conclusion HPLC is simple and stable,and can provide some scientific references for controlling the quality and evaluation of compound Jinju oral liquid.
基金项目
2022年广东省中医药局中医药科研立项项目(20222228)
维普
万方数据