Effects of YAP2 gene on rat H9c2 cardiomyocyte apoptosis and TNF-α and IL-6 levels
Objective To investigate the effect of Yes-associated protein 2(YAP2)gene silencing on rat H9c2 cardiomyocyte viability,apoptosis rate,and inflammatory factors induced by H2O2.Methods The experiment was conducted from May to December 2023.The experiment cells were divided into four groups:a normal control group,a H2O2 group,a si-YAP2+H2O2 group,and a NC+H2O2 group.MTT,flow cytometry,and Western blotting were used to detect the cell viability,apoptosis rate,and the expressions of YAP2,Bcl-2,Bax,and p-AKT protein in the four groups,and the contents of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in the cell culture medium of each group were detected by enzyme-linked immunosorbent assay(ELISA)kit.One-way analysis of variance and SNK-q test were used.Results The expression levels of YAP2 in the H2O2 group and the si-YAP2+H2O2 group were(0.716±0.056)and(0.204±0.018),respectively,with a statistically significant difference(P＜0.05).The viability of H9c2 cardiomyocytes in the H2O2 group was significantly lower than that in the si-YAP2+H2O2 group and the normal control group,while the apoptosis rate was significantly higher,with statistically significant differences(all P＜0.05).There were no statistically significant differences in the viability or apoptosis rate of H9c2 cardiomyocytes between the H2O2 group and the NC+H2O2 group(both P＞0.05).The expression levels of Bcl-2 and p-AKT in the H2O2 group were significantly lower than those in the si-YAP2+H2O2 group and the normal control group,while the expression level of Bax was significantly higher,with statistically significant differences(all P＜0.05).There were no statistically significant differences in the expression levels of Bcl-2,p-AKT,and Bax between the H2O2 group and the NC+H2O2 group(all P＞0.05).The contents of TNF-α and IL-6 in the H2O2 group were significantly higher than those in the si-YAP2+H2O2 group and the normal control group(all P＜0.05).There were no statistically significant differences in the contents of TNF-α and IL-6 between the H2O2 group and the NC+H2O2 group(both P＞0.05).Conclusion Silencing the expression of YAP2 gene may reduce the occurrence of cardiovascular diseases by reducing the apoptosis of cardiomyocytes and the contents of inflammatory factors TNF-α and IL-6.
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