Relationships between peripheral blood miR-233-3p,miR-145,and NLRP3 inflammasome and coronary artery lesions in children with Kawasaki disease
J Objective To investigate the relationships between peripheral blood miR-233-3p,miR-145,and NLRP3 inflammasome and coronary artery lesions(CAL)in children with Kawasaki disease(KD).Methods The general data of 96 children with KD treated in Children's Hospital Affiliated to Zhengzhou University from November 2021 to December 2022 were retrospectively analyzed.All the children underwent cardiac color ultrasound examination after admission.According to the KD related diagnostic criteria proposed by the American Heart Association in 2017,96 children with KD were divided into a CAL group(32 cases)and a non-CAL group(64 cases).The general data[gender,age,body mass index(BMI),fever time,etc.)and peripheral blood miR-233-3p,miR-145,and NLRP3 inflammasome levels were compared between the two groups.The multivariate logistic regression analysis was used to analyze the independent factors affecting the occurrence of CAL in KD children,and the assessment model of risk was established.The receiver operating characteristic curve(ROC)was used to evaluate the diagnostic efficiency of the model.Independent sample t test and x2 test were used.Results In the CAL group,there were 18 boys and 14 girls,aged(3.63±0.54)years,with body mass index of(13.77±2.06)kg/m2 and duration of fever of(5.41±0.81)d.In the non-CAL group,there were 35 boys and 29 girls,aged(3.58±0.53)years,with body mass index of(13.82±2.07)kg/m2 and duration of fever of(5.36±0.80)d,with no statistically significant difference(all P＞0.05).The levels of miR-233-3p and NLRP3 in the CAL group were higher than those in the non-CAL group[(1.28±0.19)vs.(0.93±0.13),(5.44±0.81)vs.(3.63±0.54)],but the level of miR-145 was lower than that in the non-CAL group[(1.07±0.16)vs.(1.98±0.29)],with statistically significant differences(t=10.606,13.027,and 16.510,all P＜0.001).Multivariate analysis by logistic regression model showed that miR-233-3p(OR=7.338,P=0.023),miR-145(OR=0.590,P=0.001),and NLRP3 inflammasome(OR=3.111,P=0.012)levels were independent influencing factors for the occurrence of CAL in KD children.According to the ROC analysis,the area under the curve(AUC)value of miR-233-3p,miR-145,and NLRP3 combined detection mode was the highest,which was 0.899,and the sensitivity and specificity were 93.75％and 90.62％,respectively.Conclusions miR-233-3p,miR-145,and NLRP3 inflammasome in peripheral blood of children with KD are closely related to the occurrence of CAL.Among them,the levels of miR-233-3p,miR-145,and NLRP3 inflammasome play an important role in the occurrence of CAL in KD children,and the combined detection of miR-233-3p,miR-145,and NLRP3 inflammasome has high clinical application value in evaluating the occurrence of CAL in KD children.
万方数据
维普
