Relationship between peripheral blood TNFα/NF-κB/NEAT1 signaling pathway and chronic pain in patients with temporomandibular joint disorder
Objective To investigate the relationship between the peripheral blood tumor necrosis factor alpha(TNFα)/nuclear factor-κB(NF-κB)/long-stranded noncoding RNA nuclear enriched transcript 1(NEAT1)signaling pathway and chronic pain in patients with temporomandibular disorder(TMD).Methods Eighty-two patients with TMD admitted to Department of Stomatology,73rd Group Army Hospital of People's Liberation Army of China from October 2022 to October 2023 were selected as a study group,including 35 males and 47 females who were(33.69±9.84)years old.According to the pain scores,the patients were divided into a mild pain group(36 cases),a moderated pain group(27 cases),and a severe pain group(19 cases).The levels of TNF-α mRNA,NF-κB mRNA,and NEAT1 mRNA in peripheral blood were compared between the 3 groups.The correlations of the levels of TNF-α mRNA,NF-κB mRNA,and NEAT1 mRNA with the score of Visual Analogue Scale(VAS)were analyzed by the Pearson analysis.Fifty-one healthy oral examinees during the same period were selected as a control group,including 29 males and 22 females who were(34.25±7.14)years old.The baseline data and levels of TNF-α,NF-κB,and NEAT1 were compared between the study group and the control group.t test,x2 test,and analysis of variance were used.The relationship of levels of TNF-α mRNA,NF-κB mRNA,and NEAT1 mRNA with TMD was analyzed by multivariate logistic regression analysis.Results The levels of TNF-α mRNA,NF-κB mRNA,and NEAT1 mRNA in the severe pain group were(0.93±0.11),(0.88±0.12),and(3.14±0.57);the levels in the moderate pain group were(0.76±0.14),(0.64±0.17),and(2.63±0.66);the levels in the mild pain group were(0.61±0.16),(0.53±0.15),and(1.96±0.59);there were statistical differences(F=31.672,33.514,and 24.976;all P＜0.05).The Pearson correlation analysis showed that the levels of TNF-α mRNA,NF-κB mRNA,and NEAT1 mRNA were positively correlated with the score of VAS(r=0.273,0.260,and 0.245;all P＜0.05).The proportions of the ones with psychological problems and poor sleep quality and the levels of TNF-α mRNA,NF-κB mRNA,and NEAT1 mRNA in the study group were higher than those in the control group[47.56％(39/82)vs.21.57％(11/51),69.51％(57/82)vs.39.22％(20/51),(0.73±0.14)vs.(0.36±0.08),(0.65±0.15)vs.(0.28±0.07),and(2.45±0.61)vs.(1.17±0.18)],with statistical differences(y2=9.055 and 11.840;t=17.193,16.515,and 14.578;all P＜0.05).The multivariate logistic regression analysis showed that psychological problems,sleep quality,TNFα mRNA,NF-κB mRNA,and NEAT1 mRNA were associated with TMD(all P＜0.05).Conclusion The peripheral blood TNFα/NF-κB/NEAT1 signaling pathway is positively correlated with pain level in patients with TMD,and may be involved in the development of TMD.
万方数据
维普
