Objective To explore the mechanism of action of Sophora flavescens lotion in the treatment of mixed hemorrhoids.Methods The drugs'main active ingredients and disease targets were screened from online pharmacology databases;enrichment analysis and molecular docking were conducted;enzyme-linked immunosorbent assay(ELISA)was used to detect the serum levels of interleukin-6(IL-6)and transforming growth factor-β(TGF-β)in 100 patients with mixed hemorrhoids before and after the treatment with Sophora flavescens lotion at Shanghang County Traditional Chinese Medicine Hospital from January 1 to December 31,2023.t test was used.Results The main active ingredients of Sophora flavescens lotion are quercetin,luteolin,β-sitosterol,stigmasterol,and kaempferol.Sophora flavescens lotion interacts with the core targets of mixed hemorrhoids,such as protein kinase B(AKT1),interleukin-1β(IL-1 β),IL-6,tumor necrosis factor(TNF),matrix metalloproteinase-9(MMP9),and TGF-β.The main active ingredients can effectively bind to the core targets.The enrichment analysis showed that it is mainly related to signaling pathways such as advanced glycation end product-receptor of advanced glycation end product(AGE-RAGE),phosphatidylinositol 3 kinase-v-akt murine thymoma viral oncogene homolog(PI3K-Akt),etc.The results of ELISA showed that the serum IL-6 level was lower after than before the treatment[10.15(3.80,14.48)vs(19.30(8.68,27.00)mg/L)mg/L],and the TGF-β level was higher after than before the treatment[0.8(0.6,1.0)mg/L vs.0.4(0.2,0.6)mg/L](both P＜0.05).Conclusions The effective active ingredients of Sophora flavescens lotion bind to the core targets,and exert therapeutic effects regulating the expression of inflammatory factors through signaling pathways such as AGE-RAGE and PI3K-Akt.This may be the main mechanism of Sophora flavescens lotion in treating mixed hemorrhoids.
Mixed hemorrhoidsSophora flavescens lotionNetwork pharmacologyMechanism of action
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