Exploration based on network pharmacology and clinical testing for mechanism of action of Sophora flavescens lotion in treatment of mixed hemorrhoids
Objective To explore the mechanism of action of Sophora flavescens lotion in the treatment of mixed hemorrhoids.Methods The drugs'main active ingredients and disease targets were screened from online pharmacology databases;enrichment analysis and molecular docking were conducted;enzyme-linked immunosorbent assay(ELISA)was used to detect the serum levels of interleukin-6(IL-6)and transforming growth factor-β(TGF-β)in 100 patients with mixed hemorrhoids before and after the treatment with Sophora flavescens lotion at Shanghang County Traditional Chinese Medicine Hospital from January 1 to December 31,2023.t test was used.Results The main active ingredients of Sophora flavescens lotion are quercetin,luteolin,β-sitosterol,stigmasterol,and kaempferol.Sophora flavescens lotion interacts with the core targets of mixed hemorrhoids,such as protein kinase B(AKT1),interleukin-1β(IL-1 β),IL-6,tumor necrosis factor(TNF),matrix metalloproteinase-9(MMP9),and TGF-β.The main active ingredients can effectively bind to the core targets.The enrichment analysis showed that it is mainly related to signaling pathways such as advanced glycation end product-receptor of advanced glycation end product(AGE-RAGE),phosphatidylinositol 3 kinase-v-akt murine thymoma viral oncogene homolog(PI3K-Akt),etc.The results of ELISA showed that the serum IL-6 level was lower after than before the treatment[10.15(3.80,14.48)vs(19.30(8.68,27.00)mg/L)mg/L],and the TGF-β level was higher after than before the treatment[0.8(0.6,1.0)mg/L vs.0.4(0.2,0.6)mg/L](both P＜0.05).Conclusions The effective active ingredients of Sophora flavescens lotion bind to the core targets,and exert therapeutic effects regulating the expression of inflammatory factors through signaling pathways such as AGE-RAGE and PI3K-Akt.This may be the main mechanism of Sophora flavescens lotion in treating mixed hemorrhoids.
Mixed hemorrhoidsSophora flavescens lotionNetwork pharmacologyMechanism of action
万方数据
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