Silencing lncRNA PSMB8-AS1 affects malignant biological behaviors of thyroid cancer cells regulating miR-144-5p expression
Objective To investigate the effects of long noncoding RNA(lncRNA)PSMB8-AS1 on the proliferation and migration of thyroid cancer cells by targeting miR-144-5p.Methods The study was conducted from March 2023 to May 2024 at the Scientific Research Center of the Affiliated Hospital of Qingdao University.The GeneCards database was used to analyze the expression of PSMB8-AS1 in thyroid cancer tissue.The real-time quantitative polymerase chain reaction(qPCR)was used to detect the expressions of PSMB8-AS1 in human normal thyroid epithelial cells HTori-3 and thyroid cancer cell lines(8505C,FTC-133,KTC-1,and TPC-1).The target gene of PSMB8-AS1 was predicted using the LncRBase and Cancer LncRNA Census databases and verified by the dual luciferase gene reporter assay.The FTC-133 cells were divided into a si-NC group and a si-PSMB8-AS1 group according to the different transfectants.The CCK-8 method and scratch assay were used to detect the effects of low-expression PSMB8-AS1 on the proliferation and migration of the FTC-133 cells.qPCR was used to detect the effects of low-expression PSMB8-AS1 on the expression of miR-144-5p and ITGA3 mRNA in the FTC-133 cells.Western blot was used to detect the effect of low-expression PSMB8-AS1 on the expression of ITGA3,Cyclin A,CDK2,FOXC2,and Hedgehog proteins in the FTC-133 cells.Results The expression of PSMB8-AS1 in the thyroid cancer tissue was higher than that in the adjacent tissue(P＜0.01).The expressions of PSMB8-AS1 in the 8505C,FTC-133,KTC-1,and TPC-1 cells were higher than that in the HTori-3 cells(all P＜0.01).PSMB8-AS1 could target and bind to miR-144-5p(P＜0.01).The expression of PSMB8-AS1 in the FTC-133 cells in the si-PSMB8-AS1 group was lower than that in the si-NC group[(1.07±0.54)vs.(6.69±1.16);t=8.78;P＜0.01].The migration rate of FTC-133 cells in the si-PSMB8-AS1 group was lower than that in the si-NC group[(27.89±6.01)％vs.(68.08±6.16)％;t=4.67;P＜0.01].The expression of miR-144-5p in the FTC-133 cells in the si-PSMB8-AS1 group was higher than that in the si-NC group,and the expression of ITGA3 mRNA was lower than that in the si-NC group(t=7.45 and 3.13;both P＜0.01).Low-expression PSMB8-AS1 could inhibit the expressions of ITGA3,Cyclin A,CDK2,FOXC2,and Hedgehog proteins in the FTC-133 cells(all P＜0.01).Conclusions PSMB8-AS1 expression is significantly upregulated in thyroid cancer tissue and cell lines.Low-expression PSMB8-AS1 can inhibit the proliferation and migration of FTC-133 cells targeting and regulating miR-144-5p.
Long noncoding RNAPSMB8-AS1Thyroid cancerMiR-144-5pProliferationMigration
万方数据
维普
