Relationship between HOPX expression and efficacy of neoadjuvant chemotherapy for patients with locally advanced breast cancer
Objective To investigate the relationship of the expression of homologous domain protein homeobox(HOPX)and the methylation of HOPX in peripheral blood with the efficacy of neoadjuvant chemotherapy for patients with locally advanced breast cancer.Methods The data of 129 patients admitted to Xianyang Central Hospital were analyzed.They were(50.69±5.48)years old.There were 71 cases of high differentiation and 58 cases of low differentiation.There were 48 cases of stage Ⅱ B and 81 cases of stage Ⅲ.Eighty-seven cases had lymph node metastasis.According to the efficacy of neoadjuvant chemotherapy,they were divided into pathological complete response(pCR)and non-pCR.The expression of HOPX and the methylation level of HOPX in peripheral blood in the patients before neoadjuvant chemotherapy were detected.The relationship of HOPX expression in cancer tissue and HOPX methylation rate in peripheral blood with the pathological features of the patients with locally advanced breast cancer was analyzed.The factors influencing the efficacy of neoadjuvant chemotherapy were analyzed.The relative expression of HOPX mRNA in cancer tissue and the methylation rate of HOPX in peripheral blood in predicting the efficacy of neoadjuvant chemotherapy for the patients were analyzed.t and x2 tests were used.The Cox proportional risk model was used for the influencing factors.The receiver operating characteristic curve(ROC)was used to evaluate the prediction efficacy.Results The relative expression level of HOPX mRNA in the cancer tissue was higher than that in the adjacent tissue[(1.97±0.32)vs.(1.13±0.21);P＜0.05].The relative expression level of HOPX mRNA and the methylation rate of HOPX in peripheral blood of the patients with low differentiation,stage Ⅲ,and lymph node metastasis were higher than those of the patients with middle and high differentiation,stage Ⅱ B,and no lymph node metastasis[(2.19±0.37)vs.(1.79±0.32),(2.23±0.39)vs.(1.53±0.26),(2.14±0.35)vs.(1.62±0.28),81.03％(47/58)vs.56.34％(40/71),75.31％(61/81)vs.54.17％(26/48),and 73.56％(64/87)vs.54.76％(23/42);all P＜0.05].The Cox regression analysis showed that tumor stage(RR=5.557,95％CI 2.286-13.505),differentiation degree(RR=5.360,95％CI 2.206-13.027),relative expression level of HOPX mRNA(RR=4.455,95％CI 1.833-10.827),and HOPX methylation rate(RR=4.362,95％CI 1.795-10.602)were the factors affecting the efficacy of neoadjuvant chemotherapy for the patients(all P＜0.05).The ROC analysis results showed that the relative expression level of HOPX mRNA combined with HOPX methylation rate had AUC in predicting the efficacy of neoadjuvant chemotherapy for the patients than single one(0.894 vs.0.774 and 0.894 vs.0.768).Conclusions The relative expression level of HOPX mRNA in cancer tissue and the methylation rate of HOPX in peripheral blood of patients with locally advanced breast cancer are closely related to pathological characteristics and the efficacy of neoadjuvant chemotherapy.The combination of the two can predict the efficacy of neoadjuvant chemotherapy well.
Breast cancerHomeodomain-only protein homeoboxPathological featuresNeoadjuvant chemotherapyCurative effect
万方数据
维普
