Correlation of expression levels of TCAB1 and lncRNA DLEU2 with and clinical pathological parameters and prognosis in patients with papillary thyroid carcinoma
Objective To investigate the correlation of the expression levels of telomerase Cahalin protein 1(TCAB1)and long chain non-coding RNA(lncRNA)DLEU2 in the tissues of patients with papillary thyroid carcinoma with their clinical pathological parameters and prognosis.Methods Eighty patients with PTC treated at Shaanxi Provincial Tumor Hospital from January 2018 to January 2021 were selected,including 35 males and 45 females who were 38-78(54.65±4.75)years old.The patients'cancer tissue and adjacent tissue were collected for immunohistochemical examination.The immunohistochemical staining was used to detect the expression levels of TCAB1 and lncRNA DLEU2 in the cancer tissue and adjacent tissue.The expression levels of TCAB1 and lncRNA DLEU2 in the patients with different pathological parameters were analyzed.Spearman correlation analysis was used to analyze the correlation of TCAB1 and lncRNA DLEU2 with the clinical pathological parameters of PTC.All the patients were followed up for 3 years,and were divided into 2 subgroups based on their prognosis.Twenty patients with new metastases and recurrent lesions and who died were set as a poor prognosis group.The remaining 60 patients were set as a good prognosis group.The expression levels of TCAB1 and lncRNA DLEU2 in the two groups were analyzed.The logistic regression analysis model was used to analyze the prognostic predictive values of TCAB1 and lncRNA DLEU2 for PTC.t andx2 tests were used.Results The relative expression level of lncRNA DLEU2 and the positive rate of TCAB1 in the cancer tissue were significantly higher than those in the adjacent tissue[(1.44±0.27)vs.(0.75±0.12),72.78％(49/80)vs.14.79％(22/80),],with statistical differences(t=16.14;x2=18.46;both P＜0.05).There were statistical differences in the relative expression of lncRNA DLEU2 and TCAB1 positivity rate between the patients with different TNM stages and with and without lymph node metastases(all P＜0.05).The results of Spearman correlation analysis showed that TCAB1 was positively associated with clinical stage and lymph node metastasis(r=0.428 and 0.353;both P＜0.05),and lncRNA DLEU2 was positively associated with clinical stage and lymph node metastasis(r=0.430 and 0.401;both P＜0.05).The relative expression level of lncRNA DLEU2 and the positive rate of TCAB1 in the poor prognosis group were significantly higher than those in the good prognosis group[(1.68±0.25)vs.(1.25±0.13)and 85.00％(17/20)vs.53.33％(32/60)],with statistical differences(t=8.48 andx2=6.34;both P＜0.05).Positive expression of TCAB1 and increased relative expression of lncRNA DLEU2 were independent prognostic factors for PTC(OR=2.791,95％CI 1.457-5.745;OR=2.146,95％CI 1.525-6.795;both P＜0.001).Conclusions The expression levels of TCAB1 and lncRNA DLEU2 in the cancer tissue of PTC patients are increased,and are closely related to the occurrence and development of PTC.The expression levels of TCAB1 and lncRNA DLEU2 can be detected to predict the prognosis of patients with PTC.
万方数据
维普
