Effect of circMBOAT2 targeting miR-526b-3p on the proliferation and apoptosis of lung cancer A549 cells
Objective To investigate whether circMBOAT2 affects the proliferation and apoptosis of lung cancer A549 cells by targeting miR-526b-3p.Methods A total of 47 fresh lung cancer tissues and para-cancer tissues were collected from Shangluo Central Hospital from June 2021 to September 2023.Real-time quantitative PCR(qRT-PCR)was used to determine the expressions of circMBOAT2 and miR-526b-3p in lung cancer tissues.Lung cancer A549 cells were divided into a Con group,a si-NC group(transfected with si-NC),a si-circMBOT2 group(transfected with si-circMBOT2),a pcDNA group(transfected with pcDNA),a pcDNA-circMBOAT2 group(transfected with pcDNA-circMBOAT2),a miR-NC group(transfected with miR-NC),a miR-526b-3p group(transfected with miR-526b-3p mimics),a si-circMBOAT2+anti-miR-NC group(co-transfected with si-circMBOAT2 and anti-miR-NC),a si-circMBOAT2+anti-miR-526b-3p group(co-transfected with si-circMBOAT2 and miR-526b-3p inhibitor).Cell proliferation,apoptosis,and protein expressions of Ki-67,Bcl-2,and Bax were measured by CCK-8,flow cytometry,and Western blotting.Dual luciferase reporter gene detection was used to analyze the targeting relationship between circMBOAT2 and miR-526b-3p.t test and SNK-q test were used.Results The expression of circMBOAT2 in the lung cancer tissues was higher than that in the para-cancer tissues[(4.25±0.22)vs.(1.00±0.05)],and the expression of miR-526b-3p was lower than that in the para-cancer tissues[(0.33±0.04)vs.(1.00±0.07)],with statistically significant differences(t=98.76 and 56.97,both P＜0.05).Interference with circMBOT2 or overexpression of miR-526b-3p inhibited the proliferation and protein expressions of Ki-67 and Bcl-2 of lung cancer A549 cells,and increased apoptosis rate and Bax protein expression(all P＜0.05).circMBOAT2 targeted to regulate the expression of miR-526b-3p,and inhibition of miR-526b-3p expression reversed the effect of interfering circMBOAT2 expression on the proliferation and apoptosis of lung cancer A549 cells.Conclusion circMBOAT2 is highly expressed in lung cancer tissues,and promotes the proliferation and inhibits apoptosis of lung cancer A549 cells by targeting the expression of miR-526b-3p.
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