Network pharmacology predicting the potential active ingredients and mechanism of Scutellaria baicalensis-Polygonum cuspidatum against chronic bronchitis
Objective To predict the potential active ingredients and mechanism of Scutellaria baicalensis-Polygonum cuspidatum against chronic bronchitis(CB)by network pharmacology.Methods The active ingredients were searched in the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)with Scutellaria baicalensis and Polygonum cuspidatum as keywords,and the screening criteria were oral bioavailability(OB)≥30％and drug-like(DL)≥0.18.Using"chronic obstructive pulmonary disease"and"chronic bronchitis"as search terms,the CB disease targets were manually identified from OMIM and GeneCards databases from the establishment of the databases to April 2024.The protein-protein interaction(PPI)network was obtained by using the STRING database.David database was used for gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,and Cytoscape 3.8.0 software was used to draw"drug-component-target-map"and"component-target-pathway map".Results A total of 23 active ingredients and 258 targets of Scutellaria baicalensis-Polygonum cuspidatum were obtained through TCMSP data screening and supplementation of key compounds.Disease targets were retrieved in GeneCards and OMIM databases,and 668 targets were obtained after deduplication and 80 intersecting targets were obtained.The core targets including TNF,IL-6,JUN,IL-1β,AKT1,VEGFA,TP53,CASP3,IL-10,and CXCL8 were determined by PPI network analysis.GO functional enrichment analysis showed that the biological processes of anti-CB of Scutellaria baicalensis-Polygonum cuspidatum mainly involved inflammatory response,oxidative stress,apoptosis,angiogenesis,and autophagy.KEGG pathway enrichment analysis showed that the anti-CB pathway of Scutellaria baicalensis-Polygonum cuspidatum might involve PI3K-AKT signaling pathway,TNF signaling pathway,IL-17 signaling pathway,cellular senescence,and so on.Conclusion The potential active ingredients of Scutellaria baicalensis-Polygonum cuspidatum anti-CB include scutellarin,baicalin,wogonin,polydatin,and emodin,and the mechanism of action may play a therapeutic role by regulating PI3K-AKT signaling pathway,TNF signaling pathway,and IL-17 signaling pathway,thereby reducing inflammatory response,improving apoptosis,and promoting autophagy.
Chronic bronchitisScutellaria baicalensis-Polygonum cuspidatumMechanism of actionNetwork pharmacology
万方数据
维普
