Mechanism of peony skin for improving hectic fever in rats with perimenopause syndrome based on hemorheology and calcitonin gene related peptide
Objective To explore the mechanism of peony skin for improving hectic fever in rats with perimenopause syndrome based on hemorheology indicators and calcitonin gene related peptide(CGRP).Methods From September to December 2022,the basic research was did at Experimental Animal Center,Fujian University of Traditional Chinese Medicine.Forty female rats were divided into a sham operation group,a model group,a peony skin group,and a Qinggusan-positive group,with 10 in each group.Except for the sham operation group,the other 3 groups underwent bilateral ovariectomy;L-thyroxine(92 mg/kg)was given once a day,for 7 consecutive days starting on the 7th day after the operation to establish Yin-deficient perimenopause syndrome models.The sham operation group and the model group were orally administered an equal amount of distilled water;the peony skin group and the Qinggusan-positive group were orally administered peony skin(2.2 g/kg)and Qinggusan(7.3 g/kg),respectively,once a day,for a total of 14 d.One hour after the last administration,the rats were anesthetized,and their blood was collected;the hemorheology indicators were detected;the serum CGRP was detected by enzyme-linked immunosorbent assay(ELISA);the expression of receptor activity-modifying protein 1(RAMP1)and calcitonin receptor-like receptor(CLR)mRNA in the rats'hypothalamuses were detected by reverse transcription-polymerase chain reaction(RT-PCR).The one-way analysis of variance was used for the statistical analysis.Results The erythrocyte sedimentation rate,hematocrit,low-,medium-,and high-shear whole blood viscosities,content of serum CGRP,and the expression levels of hypothalamic RAMP1 and CLR mRNA in the model group were higher than those in the sham operation group(all P＜0.05),and were significantly reversed by peony skin,with statistical differences(all P＜0.05).Conclusion Peony skin can improve the levels of CGRP,its receptor,and hemorheology in rats with perimenopause syndrome.
Peony skinPerimenopause syndromeHectic feverHemorheologyCalcitonin gene related peptide
万方数据
维普
