Changes and clinical significance of serum levels of miR-135b-5p and miR-126-3p in pregnant women with preeclampsia
Objective To investigate the changes of the serum levels of microRNA-135b-5p(miR-135b-5p)and microRNA-126-3p(miR-126-3p)in pregnant women with preeclampsia(PE)and their clinical significance.Methods Adopting a retrospective study.From February 2020 to February 2022,89 patients with PE treated at Second Hospital,Shaanxi University of Chinese Medicine were selected as a study group who were(27.28±2.94)years old and whose body mass index(BMI)was(22.72±2.45)kg/m2;89 normal pregnant women were selected as a control group who were(26.49±2.76)years old and whose BMI was(22.84±2.12)kg/m2.The patients were divided into a mild group(58 cases)and a severe group(31 cases)according to the severity of the disease.The patients were divided into a good group(52 cases)and a adverse group(37 cases)according to the adverse pregnancy outcomes a such as intrauterine distress,premature delivery,neonatal asphyxia,fetal death,low weight infants,macrosomia,etc.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was applied to detect the expression levels of miR-135b-5p and miR-126-3p.t and x2 tests were performed to compare the data between the two groups.Pearson correlation was carried out to analyze the correlation of the serum levels of miR-135b-5p and miR-126-3p with systolic blood pressure,diastolic blood pressure,and proteinuria in the patients.Multivariate logistic regression was used to analyze the factors affecting PE degree.The diagnostic value of the patients with severe PE and the predictive value of adverse pregnancy outcomes in the patients were analyzed using the receiver operating characteristic curve(ROC).Results The serum levels of miR-135b-5p and miR-126-3p in the study group were lower than those in the control group(0.69±0.14 vs.1.07±0.23 and 0.65±0.13 vs.1.08±0.25),with statistical differences(both P＜0.05).The serum levels of miR-135b-5p and miR-126-3p in the study group were lower than those in the mild group(0.49±0.11 vs.0.79±0.15 and 0.53±0.12 vs.0.71±0.14),with statistical differences(both P＜0.05).The serum levels miR-135b-5p and miR-126-3p in the good group were lower than those in the adverse group(0.53±0.12 vs.0.81±0.18 and 0.52±0.11 vs.0.75±0.15),with statistical differences(both P＜0.05).The Pearson correlation analysis showed that of miR-135b-5p was negatively correlated with systolic blood pressure,diastolic blood pressure,and proteinuria(r=-0.492,-0.612,and-0.522;all P＜0.05);miR-126-3p was negatively correlated with systolic blood pressure,diastolic blood pressure,and proteinuria(r=-0.519,-0.481,and-0.626;all P＜0.05).Multivariate logistic regression analysis revealed that low levels of miR-135b-5p and miR-126-3p were risk factors for severe PE(both P＜0.05).According to the ROC analysis,the areas under the curves(AUC)of the combination of miR-135b-5p and miR-126-3p for the diagnosis of the patients with severe PE and the prediction of adverse pregnancy outcomes were 0.948 and 0.952,respectively,which were higher than those of either indicator(both P＜0.05).Conclusion The expression levels of miR-135b-5p and miR-126-3p in the serum of patients with PE decrease,and are related to the development of PE and adverse pregnancy outcomes.The combination of the two has high diagnostic value for patients with severe PE and predictive value for adverse pregnancy outcomes.
万方数据
维普
