染料木素磺酸钠减轻慢性脑缺血大鼠神经细胞焦亡的研究
The effects of Genistein-3'-sodium sulfonate on the cortex of rats with chronic cerebral ischemia by inhibiting pyroptosis
邢亚康 1张丽梅 1王恺迪 1温亚娟 2黎晓 3帅萍2
作者信息
- 1. 赣南医科大学基础医学院
- 2. 赣南医科大学第一附属医院病理科
- 3. 赣南医科大学基础医学院;赣南医科大学心脑血管疾病防治教育部重点实验室,江西 赣州 341000
- 折叠
摘要
目的:探讨染料木素磺酸钠(Genistein-3'-sodium sulfonate,GSS)对慢性脑缺血大鼠大脑皮层细胞焦亡的影响.方法:采用改良型双侧颈总动脉结扎法(Bilateral carotid artery ligation,2-VO)制备慢性脑缺血大鼠模型.将大鼠随机分为假手术组(Sham)、模型组(2-VO+Vehicle)、治疗组(2-VO+GSS)3组,应用ELISA方法检测各组大鼠血清中IL-1β的活性变化;并应用Real time-PCR和Western Blot方法检测大鼠大脑皮层组织中IL-1β、NLRP3、Caspase-1、GSDMD的mRNA及(或)蛋白表达水平.结果:与假手术组相比,模型组大鼠外周血清中IL-1β含量显著升高(P=0.042 9);与模型组比较,治疗组大鼠外周血清中IL-1β含量下降,但差异无统计学意义(P=0.073 3).对各组大鼠大脑皮层组织中IL-1β的mRNA及蛋白表达水平观察发现,与假手术组比较,模型组大鼠大脑皮层IL-1β的mRNA表达水平及蛋白表达水平均升高,差异有统计学意义(P=0.025 5,P＜0.000 1);与模型组比较,治疗组大鼠大脑皮层IL-1β的mRNA表达水平及蛋白表达水平均下降,差异有统计学意义(P=0.020 0,P＜0.000 1).与假手术组比较,模型组大鼠大脑皮层中NLRP3、GSDMD和Caspase-1 P20的mRNA水平(P=0.044 3,P=0.022 9,P＜0.000 1)及蛋白表达水平(P=0.000 3,P=0.000 7,P=0.074 1)均升高;与模型组比较,治疗组脑皮层中NLRP3、GSDMD和Caspase-1 P20的mRNA水平(P=0.001 2,P=0.004 4,P=0.005 8)及蛋白表达水平(P=0.008 4,P=0.026 8,P=0.038 5)均降低.结论:GSS可通过抑制神经细胞焦亡减轻慢性脑缺血损伤.
Abstract
Objective:To investigate the effect of Genistein-3'-sodium sulfonate(GSS)on cell pyroptosis in rats with chronic cerebral ischemia.Methods:A modified bilateral common carotid artery occlusion(2-VO)method was used to establish a rat model of chronic cerebral ischemia.The rats were randomly divided into sham group(Sham),model group(2-VO+Vehicle)and treatment group(2-VO+GSS).IL-1β activity in rat serum was assessed using ELISA.The mRNA and protein expression levels of IL-1β,NLRP3,Caspase-1,and GSDMD in the cortical tissue of rats were detected by real-time PCR and Western blot.Results:Compared with the sham group,the activity of IL-1β in model group rats'serum decreased significantly(P=0.042 9),compared with model group,the activity of IL-1β in treatment group rats'serum decreased,but with no significant difference(P=0.073 3).The study of the mRNA or protein levels of IL-1β from the cerebral cortex showed,compared with sham group,the IL-1βmRNA and protein expression were increased(P=0.025 5,P＜0.000 1);compared with model group,the IL-1β mRNA and protein expression in the treatment group were decreased,with statistical significance(P=0.020 0,P＜0.000 1).Compared with sham group,the mRNA levelof NLRP3、GSDMD and Caspase-1 P20(P=0.044 3,P=0.022 9,P＜0.000 1)and protein level(P=0.000 3,P=0.000 7,P=0.074 1)in the cerebral cortex of all rats in the model group increased.Compared with model group,the mRNA level of NLRP3、GSDMDand Caspase-1 P20(P=0.001 2,P=0.004 4,P=0.005 8)and protein level(P=0.008 4,P=0.026 8,P=0.038 5)in the cerebral cortex of all rats in the treatment group were decreased.Conclusion:GSS may alleviate chronic cerebral ischemic injury by inhibiting neuronal pyroptosis.
关键词
慢性脑缺血/染料木素磺酸钠/细胞焦亡Key words
Chronic cerebral ischemia/Genistein-3'-sodium sulfonate/Pyroptosis引用本文复制引用
基金项目
国家自然科学基金项目(82160688)
赣州市指导性科技计划项目(GZ2021ZSF018)
赣州市指导性科技计划项目(2022B-SF9554)
赣南医学院本科生科技创新项目(202010)
赣南医学院神经生物研究创新团队项目(TD2021JC06)
出版年
2024
国家科技期刊平台
NSTL
万方数据