钙敏感受体在血吸虫病肝纤维化中的作用
The role of CaSR in liver fibrosis caused by schistosomiasis
张文娟 1李珂云 2冯嘉禾 2林乐迎 2熊俊 2秦静茹 3胡衡 2李珍妮 2贺喆 2曹镐禄2
作者信息
- 1. 赣南医科大学基础医学院;赣南医科大学心脑血管疾病防治教育部重点实验室
- 2. 赣南医科大学基础医学院
- 3. 赣南医科大学第一临床医学院,江西 赣州 341000
- 折叠
摘要
目的:探讨钙敏感受体(Calcium sensitive receptors,CaSR)在血吸虫病肝纤维化中的作用.方法:采用BALB/c小鼠建立血吸虫病肝纤维化模型.通过HE和Masson染色观察小鼠肝组织病理学变化,免疫组织化学、RT-qPCR和Western blot技术分别检测肝组织中CaSR和纤维化相关指标表达,ALT和AST试剂盒检测肝组织氨基转移酶含量,天狼星红和VG染色技术观察CaSR抑制剂(Calhex231)对小鼠肝纤维化的影响.结果:HE染色结果显示,未感染组小鼠肝细胞排列整齐,感染组小鼠肝组织中出现大量虫卵且虫卵周围有胶原纤维沉积,形成虫卵肉芽肿,Masson染色结果显示,未感染组小鼠肝细胞排列整齐,肝组织未见明显胶原沉积,感染组小鼠肝组织大量破坏,出现大量假小叶且周围有大量胶原纤维化增生;与未感染组相比,感染组小鼠肝组织α-SMA、Collagen-Ⅰ和CaSR表达增加,ALT和AST含量增加,差异均有统计学意义(P＜0.05);天狼星红染色结果显示,感染组小鼠肝组织结构已经严重破坏,且出现大量胶原纤维沉积;CaSR抑制剂干预组小鼠肝组织结构出现破坏,但尚有部分组织为完整结构;VG染色结果显示,感染组小鼠肝组织已呈现出大量粉红色,而CaSR抑制剂干预组小鼠肝组织呈现出部分粉红色,尚有部分完整组织结构.结论:CaSR可能在血吸虫病肝纤维化中发挥作用.
Abstract
Objective:To investigate the role of calcium sensitive receptorsin schistosomiasis liver fibrosis.Methods:The BALB/c mice were used to establish a liver fibrosis model of schistosomiasis.The HE and Masson staining were used to observe pathological changes in mouse liver tissues.The Immunohistochemistry,RT-qPCR,and Western blot techniques were used to detect the expression of CaSR and fibrosis related indicators in the liver tissues.The ALT and AST assay kits were used to detect the content of liver tissue aminotransferase.The Sirius Red and VG staining were used to observe the effect of CaSR inhibitor(Calhex231)on liver fibrosis in mice.Results:The HE staining was found that the liver cells of the uninfected group mice were arranged neatly,while a large number of the eggs were found in the liver tissue of the infected group mice,with collagen fibrosis deposition around the eggs,forming granulomas of the eggs.The Masson staining results showed that the liver cells of the uninfected group mice were arranged neatly,and no obvious collagen deposition was observed in the liver tissues.While the liver tissue of the infected group mice was extensively damaged,with a large number of pseudolobules and a large amount of collagen fibrosis proliferation around pseudolobules.Compared with the uninfected group,the expressions of α-SMA,Collagen-Ⅰ,and CaSR in the liver tissue of the infected group mice were increased,and the contents of ALT and AST increased,and the differences were statistically significant(P＜0.05).The Sirius Red staining results showed that the liver tissue structure of the infected mice had been severely damaged,and a large amount of collagen fibrosis deposition had occurred;the CaSR inhibitor intervention group showed that damage to the liver tissue structure of the mice,but there were still some tissues with complete structures.The VG staining results showed that the liver tissue of the infected group mice had already shown a large amount of pink,while the liver tissue of the CaSR inhibitor intervention group mice showed partial pink,with some complete tissue structures.Conclusion:CaSR may play a role in schistosomiasis liver fibrosis.
关键词
日本血吸虫/肝纤维化/钙敏感受体Key words
Schistosoma japonicum/Liver fibrosis/Calcium-sensitive receptor引用本文复制引用
基金项目
江西省教育厅科学技术研究项目(GJJ2201410)
出版年
2024
国家科技期刊平台
NSTL
万方数据