纳米孔基因测序技术在HLA基因分型中的应用
Application of nanopore sequencing technology in HLA typing
朱孟琳 1何燕琴 2谢水莲 1杨影 1万绍贵3
作者信息
- 1. 赣南医科大学基础医学院
- 2. 赣州市中心血站检验科
- 3. 赣南医科大学基础医学院分子病理中心,江西 赣州 341000
- 折叠
摘要
人类白细胞抗原(Human leukocyte antigen,HLA)基因是人类基因组中最复杂的基因区域,主要参与机体免疫反应,HLA的基因分型在器官移植以及疾病诊疗等领域有着广泛的临床应用.现有的HLA分型方法在临床实践中发挥了重要作用,但也存在一些技术限制和局限性.纳米孔基因测序技术的出现为快速鉴定HLA基因分型提供了全新视角.该技术以其快速、实时测序和长读长等特点,为复杂的HLA区域提供了更为快速精确的识别能力.该技术不仅能更快速地解析HLA基因型,还对前沿高通量基因测序新技术开发HLA分型方法的研究具有潜在的借鉴意义.本文主要对纳米孔基因测序技术在HLA分型领域中的研究方法和临床应用进行综述.
Abstract
The human leukocyte antigen(HLA)gene is the most complex regions of the human genome,which mainly participates in the immune response of the body.HLA typing has extensive clinical applications in organ transplantation,disease diagnosis and treatment,and other related fields.The existing methods for HLA typing have played a crucial role in clinical practice,but they do have certain technical limitations.However,the advent of nanopore sequencing technol-ogy has offered a fresh perspective for the rapid identification of HLA typing.This technology,with its fast and real-time sequencing capabilities,as well as its long read length characteristics,facilitates faster and more accurate recognition of complex HLA regions.It not only enables quicker analysis of HLA genotypes,but also has the potential to serve as a ref-erence for the development of cutting-edge high-throughput gene sequencing methods for HLA typing.This article mainly reviews the research methods and clinical applications of nanopore gene sequencing technology in the field of HLA typing.
关键词
人类白细胞抗原/基因分型技术/纳米孔基因测序/器官移植Key words
Human leukocyte antigen/Genotyping techniques/Nanopore sequencing/Organ transplantation引用本文复制引用
基金项目
赣南医学院校级课题(YC2022-X012)
出版年
2024
国家科技期刊平台
NSTL
万方数据