基于非靶向代谢组学探讨不同体型成年犬的代谢特征及差异
Study on the metabolic characteristics and differences of adult canines of different sizes based on untargeted metabolomics
李晏姗 1梁梦芸 1詹艳 1刘媛媛 1刘典雨 1陈芸 1程旭 2胡宇峰2
作者信息
- 1. 赣南医科大学赣南创新与转化医学研究院,重大疾病新药靶发现及新药创制全国重点实验室
- 2. 赣南医科大学赣南创新与转化医学研究院,重大疾病新药靶发现及新药创制全国重点实验室;赣南医科大学第一附属医院,江西 赣州 341000
- 折叠
摘要
目的:探讨不同体型犬之间的代谢特征及差异,为不同体型犬提供更好的健康指导及疾病预防.方法:收集大型犬及小型犬的新鲜血清及血浆样本,部分血液样本进行血常规分析,部分样本在离心分离血浆后进行血生化及非靶向代谢组学检测分析不同体型犬的代谢特征.通过偏最小二乘判别分析(Partial least squares discriminant analysis,PLS-DA)构建模型,结合模型及变量重要性投影(Variable importance in projection,VIP)筛选差异代谢物.结果:大型犬及小型犬血清生化指标如谷草转氨酶(Aspartate aminotransferase,AST)、总胆红素(Total bilirubin,TBil)、直接胆红素(Direct bilirubin,DBil)、间接胆红素(Indirect bilirubin,IBil)、高密度脂蛋白胆固醇(High density lipoprotein cholesterol,HDL-C)这5种指标差异有统计学意义(P＜0.05).采用血浆非靶向代谢组学分析鉴定出了不同体型犬血浆中存在1 277种代谢物,具体分为脂质和类脂质分子,有机酸及其衍生物,杂环有机化合物,苯及其衍生物,有机含氧化合物,苯丙烷类化合物和多酮类化合物,有机氮化合物,核苷、核苷酸和类似物,碱和衍生物9类.生物信息学分析表明,差异代谢物富集在不同的KEGG通路,这些通路包括嘧啶代谢、色氨酸代谢、氨酰-tRNA生物合成、次级胆汁酸生物合成以及精氨酸和脯氨酸代谢.其中氨酰-tRNA生物合成通路最显著且呈上调趋势,因此该通路及通路中的显著差异代谢物L-苯丙氨酸和L-赖氨酸可作为区分大小型犬代谢差异的重要靶点.结论:本研究揭示了不同体型犬之间的代谢差异,为不同体型犬的代谢特征提供了更准确的信息,有助于进一步了解不同体型犬的代谢特征,有助于更科学地饲养和管理.
Abstract
Objective:To explore the metabolic characteristics and differences among canines with different body sizes,and then to provide better health guidance and disease prevention for canines with different body sizes.Methods:By collecting fresh serum and plasma samples from canines with different body sizes,some blood samples for routine blood analysis,and some samples for blood biochemical and untargeted metabolomics tests to explore the metabolic characteristics of canines with different body sizes.To develop a robust model utilizing partial least squares discriminant analysis(PLS-DA)and employ variable importance in projection(VIP)scores as a means of identifying significant differential.Results:There are significant discrepancies in key serum biochemical markers,including aspartate aminotransferase(AST),total bilirubin(TBil),direct bilirubin(DBil),indirect bilirubin(IBil),and high-density,lipoprotein choles-terol(HDL-C).By using plasma untargeted metabolomics analysis,it identified the canine plasma 1 277 metabolites and divided into 9 categories,lipids and lipid-like molecules,organic acids and derivatives,organic heterocyclic compound,benzoic acids,organic oxides,phenylpropane and polyketones,organic nitrogen compounds,nucleosides and nucleo-tides and analogues,alkaloids and derivatives.Bioinformatics analysis indicated that differential metabolites were en-riched in different KEGG pathways including pyrimidine metabolism,tyrosine metabolism,aminoacyl-tRNA biosynthe-sis,secondary bile acid biosynthesis,as well as arginine and proline metabolism.Among them,the aminoacyl-tRNA biosynthesis pathway was the most significant and wasupregulated.Therefore,the significant differential metabolites L-phenylalanine and L-lysine in this pathway can be used as important targets to distinguish metabolic differences in canine size and body size.Conclusion:This study reveals the metabolic differences between canines with different body sizes,providing more accurate information on the metabolic characteristics of canines with different body sizes.These data can also help us further understand the metabolic characteristics of canines with different body sizes and contribute to more scientific breeding and management.
关键词
犬类/体型/代谢特征/非靶向代谢组学分析Key words
Canine/Body size/Metabolic characteristics/Untargeted metabolomic analysis引用本文复制引用
基金项目
国家自然科学基金(82300684)
江西省科技厅重点研发项目(20223BBG71008)
赣州市章贡区科技重大专项()
出版年
2024
国家科技期刊平台
NSTL
万方数据