Mechanism Study on Screening Heterotrophic Nitrifying Bacteria and Microbial Community Restoration of Black-odorous Water
Twenty-eight heterotrophic nitrifying bacteria were screened and purified from the sediment of a black and odorous water body in a rural area,and 5 dominant bacterial strains with better pollutant removal efficiency were selected for strain recombination research.Through 16S rDNA sequencing and homology comparison,heterotrophic nitrifying bacteria X-1 and X-25 are both Pseudomonas sp.During operation,heterotrophic nitrifying bacteria group 2(X-1 and X-25)had a more stable removal efficiency for COD,organic pollutants(DOM),and ammonia nitrogen.The maximum removal rate of COD reached 41.23%,and the maximum removal rate of ammonia nitrogen could reach 83.26%.The increase in peak A and peak B of protein like fluorescence was significant,and the optimal inoculation amount of heterotrophic nitrifying bacteria group was 0.001%Heterotrophic nitrifying bacteria have synergistic effects and microbial physiological characteristics,which will have greater potential for application in the restoration of black-odorous water.
black-odorous water restorationheterotrophic nitrifying bacteriaCODammonia nitrogendissolved organic matter