首页|马铃薯StSnRK2.4基因的克隆及其序列特征分析

马铃薯StSnRK2.4基因的克隆及其序列特征分析

Cloning and analysis of characteristics sequence of StSnRK2 .4 gene in potatoes

扫码查看
利用 RT-PCR技术从马铃薯普通栽培品种‘陇薯3号’试管苗根部扩增得到StSnRK2.4基因的 cDNA序列,并对其编码蛋白进行特性和结构分析.结果表明:该基因序列全长1083 bp,编码360个氨基酸,与烟草SnRK2家族基因具有较高的同源性,达95.56%,已注册该基因到 GenBank(No.JX280914);该蛋白分子量为41.49 kU,理论等电点为5.52,是一个不跨膜的膜内蛋白,主要存在于细胞核中,含有依赖 cAMP/cGMP 蛋白激酶磷酸化、蛋白激酶C磷酸化、酪蛋白激酶Ⅱ磷酸化、酪氨酸蛋白激酶磷酸化和豆蔻酰化位点,丝氨酸/苏氨酸蛋白激酶活性位点信号序列和蛋白激酶 ATP结合区域信号序列,推测该基因功能与植物抗逆境胁迫有关.
The cDNA sequence of StSnRK2.4 were cloned by RT-PCR from the test-tube seedling roots of common potato cultivars ‘Longshu-3’.The full sequence contained 1 083 bp and encoded 360 ami-no acids,and there were high homologies with the SnRK2 gene family of tobacco,and the homology was 95.56%.The gene was registered to GenBank (No.JX280914).The bioinformatics analysis showed that the molecular weight of SnRK2.4 was 41.49 kU,the theoretical isoelectric point was 5.52,and it had not trans-membrane domain and mainly existed in the cell nucleus containing some active sites,such as cAMP-and cGMP-dependent protein kinase phosphorylation site,Protein kinase C phosphorylation site,Casein ki-nase II phosphorylation site,Tyrosine kinase phosphorylation site and N-myristoylation site,Serine/Threo-nine protein kinases active-site and protein kinases ATP-binding region.It is suggested that the function of the gene can associate with plant resistance to adversity stress.

SolanumtuberosumStSnRK2.4 genecloningbioinformatics analysisstress tolerance

李洋、白江平、毛娟、王丽、范阿棋、张俊莲、王蒂

展开 >

甘肃省作物遗传改良与种质创新重点实验室,甘肃农业大学农学院,甘肃 兰州 730070

甘肃省干旱生境作物学重点实验室,甘肃农业大学生命科学技术学院,甘肃 兰州 730070

马铃薯 StSnRK2.4基因 克隆 生物信息学分析 抗逆性

国家科技支撑计划甘肃省重大专项项目

2012BAD06B031102NKDA025

2014

甘肃农业大学学报
甘肃农业大学

甘肃农业大学学报

CSCD北大核心
影响因子:0.612
ISSN:1003-4315
年,卷(期):2014.(2)
  • 1
  • 9