1-aminocyclopropane-1-carboxlic acid synthase (ACS)is a rate-limiting enzyme in pathway of ethylene biosynthesis.To elucidate the gene structure,RNA was extracted from leaves of the potato culti-var ‘Gannongshu 2’to design gene special primers.The target gene ACS was cloned by RT-PCR,and the target gene and protein were analyzed by bioinformatics.The results showed that CDS of ACS gene con-tained 1 461 bp nucleotides which encoded 486 amino acids with molecular weight of 55 kU,PI 6.76.ACS had 12 alpha helical regions,22 beta sheet regions,32 corners and 23 free-curl zones and had 39 hydropho-bic regions and 41 hydrophilic regions.The results from homology analysis showed that ACS highly con-served in higher plants.
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