摘要
[目的]研究贯叶金丝桃提取物(Hypericum perforatum extract,HPE)对急性缺氧大鼠肝肾损伤的改善作用.[方法]通过小鼠常压密闭实验模型初步筛选HPE(20%乙醇提取物、70%乙醇提取物和95%乙醇提取物)的抗缺氧活性组分.进一步建立大鼠低压低氧肝、肾损伤模型:将80只健康大鼠随机分为8组,分别为常氧空白组(NG),缺氧模型组(HG),HPE低、中、高剂量组(HPE-L、HPE-M、HPE-H,100、200、400 mg/kg),金丝桃苷低、高剂量组(HYP-L、HYP-H,50、100 mg/kg)和醋酸地塞米松组(DXM,4 mg/kg).观察HPE对急性缺氧大鼠肝、肾组织病理学变化的影响,并测定其中氧化应激指标和相关炎症因子的变化.[结果]与模型组比较,给予HPE干预后的大鼠肝、肾组织中丙二醛(MDA)和过氧化氢(H2O2)含量下降(P<0.01,P<0.05),谷胱甘肽(GSH)含量增加(P<0.01,P<0.05),总超氧化物歧化酶(T-SOD)和过氧化氢酶(CAT)活力有所提高(P<0.01,P<0.05).同时,大鼠肝、肾组织中炎症因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和血管内皮生长因子(VEGF)的释放均被抑制(P<0.01,P<0.05).[结论]HPE对急性缺氧大鼠肝、肾损伤具有一定程度的改善作用.
Abstract
[Objective]This study aimed to investigate the potential ameliorative effects of Hypericum perforatum L.extract(HPE)on liver and kidney injury in acute hypoxia rats.[Method]The anti-hypoxic active components of HPE,including 20%ethanol extract,70%ethanol extract,and 95%ethanol extract,were initially screened using a normobaric hypoxia model in mice.Subsequently,a hypobaric hypoxia injury model was established using 80 rats.The rats were randomly divided into various groups:normoxia control group(NG),hypoxia model group(HG),HPE low,medium,and high dose groups(HPE-L,HPE-M,HPE-H,100,200,400 mg/kg),hyperoside low and high dose groups(HYP-L,HYP-H,50,100 mg/kg),and dexamethasone group(DXM,4 mg/kg).The effects of HPE on the histopathological changes of the liver and kidney in acute hypoxia rats were observed,and related inflammatory factors and oxidative stress in-dexes were measured.[Result]Compared with the model group,rats treated with HPE showed decreased levels of malondialdehyde(MDA)and hydrogen peroxide(H2O2)(P<0.01,P<0.05),and increased levels of glutathione(GSH),total superoxide dismutase(T-SOD),and catalase(CAT)activities(P<0.01,P<0.05).Furthermore,the release of inflammatory factors interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and vascular endothelial growth factor(VEGF)was inhibited in rats treated with HPE(P<0.01,P<0.05).[Conclusion]HPE demonstrated a certain degree of ameliorative effect on liver and kidney injury in rats with acute hypoxia.
基金项目
中央高校基本科研业务费专项资金项目(31920200010)
甘肃省自然科学基金项目(20JR5RA594)
NETL
NSTL
万方数据