Ameliorative Effect of Hypericum perforatum Extract on Acute Hypoxia Rats with Injury of Liver and Kidney
[Objective]This study aimed to investigate the potential ameliorative effects of Hypericum perforatum L.extract(HPE)on liver and kidney injury in acute hypoxia rats.[Method]The anti-hypoxic active components of HPE,including 20%ethanol extract,70%ethanol extract,and 95%ethanol extract,were initially screened using a normobaric hypoxia model in mice.Subsequently,a hypobaric hypoxia injury model was established using 80 rats.The rats were randomly divided into various groups:normoxia control group(NG),hypoxia model group(HG),HPE low,medium,and high dose groups(HPE-L,HPE-M,HPE-H,100,200,400 mg/kg),hyperoside low and high dose groups(HYP-L,HYP-H,50,100 mg/kg),and dexamethasone group(DXM,4 mg/kg).The effects of HPE on the histopathological changes of the liver and kidney in acute hypoxia rats were observed,and related inflammatory factors and oxidative stress in-dexes were measured.[Result]Compared with the model group,rats treated with HPE showed decreased levels of malondialdehyde(MDA)and hydrogen peroxide(H2O2)(P<0.01,P<0.05),and increased levels of glutathione(GSH),total superoxide dismutase(T-SOD),and catalase(CAT)activities(P<0.01,P<0.05).Furthermore,the release of inflammatory factors interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and vascular endothelial growth factor(VEGF)was inhibited in rats treated with HPE(P<0.01,P<0.05).[Conclusion]HPE demonstrated a certain degree of ameliorative effect on liver and kidney injury in rats with acute hypoxia.
Hypericum perforatum L.hypoxialiver and kidney injuryoxidative stressinflammation
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