大鼻吻鮈胚胎发育特征和初孵仔鱼形态观察
Embryonic development and morphologic observations of newly-hatched Rhinogobio nasutus Larvae
杨瑞兰 1刘彦斌 2赵红雪 3刘凯 2杨立强 1肖伟 2赛清云 2田永华 2赖章龙 4阮超岭 4柳婷 1刘哲 5连总强2
作者信息
- 1. 甘肃农业大学动物科学技术学院,甘肃 兰州 730070;宁夏回族自治区水产研究所(有限公司),宁夏银川 750001
- 2. 宁夏回族自治区水产研究所(有限公司),宁夏银川 750001;宁夏渔业工程技术研究中心,宁夏银川 750001
- 3. 宁夏大学生命科学院,宁夏 银川 750021;宁夏渔业工程技术研究中心,宁夏银川 750001
- 4. 宁夏回族自治区水产研究所(有限公司),宁夏银川 750001;喀什大学生命与地理科学学院,新疆 喀什 844000
- 5. 甘肃农业大学动物科学技术学院,甘肃 兰州 730070
- 折叠
摘要
[目的]为建立大鼻吻鮈(Rhinogobio nasutus)规模化人工繁育技术,并为其生产性育苗工艺和种质资源保护等提供科学依据.[方法]采用野生的大鼻吻鮈经人工催产获得受精卵,记录并分析其胚胎及仔鱼发育各时期的形态特征.[结果]大鼻吻鮈成熟卵呈圆球形,浅绿色,为漂流性卵,卵径(0.60±0.08)mm,受精卵吸水充分膨胀后卵径(0.76±0.08)mm.在水温(23.2±1.0)℃,pH 8.71～8.86条件下,受精卵3.67 h发育至囊胚期,5.95 h发育至原肠期,9.55 h胚体形成,13.38 h器官开始形成,19.98 h出现肌肉效应情况,24.05 h心跳出现,40.43 h完成出膜,至仔鱼期.大鼻吻鮈初孵仔鱼全长3.00～4.00 mm,心跳101～132次/min;在水温26℃条件下,仔鱼2 d全部吸收完卵黄,部分泄殖孔已开,3 d全部开口摄食.[结论]利用人工催产的方法记录、分析和描述了大鼻吻鮈的胚胎发育过程,并且成功获得了大鼻吻鮈初孵仔鱼,其胚胎发育过程经历胚盘期、卵裂期、囊胚期、原肠胚期、神经胚期、器官形成期及孵化出膜7个阶段,每个发育阶段又有若干个发育时期.
Abstract
[Objective]The aim of this study was to establish large-scale artificial breeding technology and provide a scientific basis for the establishment of productive seedling and the protection of germplasm resources for Rhinogobio nasutus.[Method]Fertilized eggs of Rhinogobio nasutus were obtained through artificially-induced spawning,and the morphological characteristics of embryo and larval development were recorded and analyzed at a constant water temperature of(23.2±1.0)℃.[Result]The unfertilized mature eggs were spherical,light green,and drifting,with a diameter of(0.60±0.08)mm.After fertilization and hy-dration,the fully swollen eggs became translucent,with a diameter of(0.76±0.08)mm.Under the condi-tions of water temperature(23.2±1.0)℃ and pH 8.71～8.86,the fertilized eggs developed into the blas-tula stage in 3.67 hours,the gastrula stage in 5.95 hours,embryo body formation in 9.55 hours,organ for-mation in 13.38 hours,muscle effect in 19.98 hours,and heartbeat in 24.05 hours.After 40.43 hours,em-bryo development was completed,and the larval stage was reached.The overall length of newly hatched lar-vae ranged from about 3.00 to 4.00 mm,with a heartbeat of 101～132 beats per minute.The yolk of the lar-vae was completely absorbed within 2 days,and in some larvae,the cloaca was opened.All larvae started in-gestion 3 days after hatching,under the condition of a water temperature of 26℃.[Conclusion]This study recorded,analyzed,and described the process of embryonic development of the big-nosed minnow using arti-ficial labor induction.Hatching larvae of Rhinogobio nasutus were successfully obtained.The embryonic de-velopment process went through seven stages,including the embryo dish stage,cleavage stage,blastocyst stage,gastrula stage,neuroembryonic stage,organ formation stage,and hatching out of the membrane.Each development stage consisted of several phases.
关键词
大鼻吻鮈/胚胎发育/仔鱼/形态特征Key words
Rhinogobio nasutus/embryonic development/larval/morphological characteristics引用本文复制引用
基金项目
宁夏重点研发计划项目(2023BCF01013)
农业农村部财政专项(HHDC-2022-02)
2022年自治区重点研发计划黄河流域生态环境保护与高质量发展科技支撑专项(2022BEG02056)
出版年
2024
NETL
NSTL
万方数据