Screening and stability verification of kohlrabi reference genes
[Objective]By screening known reference genes,the most appropriate reference genes in cab-bage were identified to ensure the accurate expression of quantitative real-time PCR(QRT-PCR).[Method]Eleven reference genes were selected in this experiment,and the internal reference genes of dif-ferent parts of purple and green cabbage were analyzed by GeNorm,Normalfinder and Bestkeep software.[Result]Three analysis results showed that the internal reference gene Tip41 was expressed most stably in different tissue parts of kohlrabi.[Conclusion]Tip41 was the best choice as an internal reference gene,pro-vide a basis for the subsequent molecular biology related studies of kohlrabi.
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