Seed germination characteristics of Mandragora chinghaiensis
[Objective]To study the seed germination characteristics of Mandragora chinghaiensis.[Method]The seeds of Mandragora chinghaiensis were used as the test material,and three methods of ger-mination were selected:conventional sowing,low temperature treatment(4℃)and tissue culture.[Result]The viability of M.chinghaiensis seeds was 65%by TTC method staining.Conventional sowing and low temperature treatment failed to germinate the seeds of M.chinghaiensis,but only the seedlings were ob-tained by tissue culture.The most suitable pre-treatment for seeds was 300 mg/L of GA3 soaked for 16 h.The best disinfection method was 20%NaClO disinfection 15 min.The most suitable base medium for ger-mination is 1/2MS.The most favorable hormone combination for seed germination of M.chinghaiensis was 1/2 MS+6-BA 2 mg/L+NAA 0.1 mg/L+ GA3 0.3 mg/L.[Conclusion]By exploring the germination system of M.chinghaiensis seeds,this study can alleviate the bottleneck problem that M.chinghaiensis seed-lings are extremely difficult to obtain for artificial propagation,and provide new support for further research on its pharmacological value and biological properties.
Mandragora chinghaiensisseed germinationlow temperature treatmenttissue culturehormone combination
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