青海茄参种子的萌发特性
Seed germination characteristics of Mandragora chinghaiensis
段红俊 1代思雨 1李强峰 1苏艺芃 1李紫怡 1赵艳艳1
作者信息
- 1. 青海大学农牧学院,青海 西宁 810016
- 折叠
摘要
[目的]本试验旨在探索青海茄参种子的萌发特性.[方法]以青海茄参(Mandragora chinghaiensis)的种子为试验材料,选择常规播种,低温处理(4℃),组织培养3种方式进行萌发试验.[结果]青海茄参种子经 TTC法染色检测,活力为65%;常规播种与低温处理无法使青海茄参种子萌发,只有组织培养的方式获得了种苗;对种子的预处理最适宜的方式为300 mg/L的GA3浸泡16 h;最佳的消毒方式为20%NaClO消毒15 min;最适宜萌发的基本培养基为1/2MS;最有利于青海茄参种子萌发的激素组合为1/2 MS + 6-BA 2 mg/L + NAA 0.1 mg/L + GA3 0.3 mg/L.[结论]探索青海茄参种子的萌发体系,可以缓解青海茄参人工繁殖困难种苗极难获得的瓶颈问题,为进一步研究其药学价值及生物学特性提供新的支持.
Abstract
[Objective]To study the seed germination characteristics of Mandragora chinghaiensis.[Method]The seeds of Mandragora chinghaiensis were used as the test material,and three methods of ger-mination were selected:conventional sowing,low temperature treatment(4℃)and tissue culture.[Result]The viability of M.chinghaiensis seeds was 65%by TTC method staining.Conventional sowing and low temperature treatment failed to germinate the seeds of M.chinghaiensis,but only the seedlings were ob-tained by tissue culture.The most suitable pre-treatment for seeds was 300 mg/L of GA3 soaked for 16 h.The best disinfection method was 20%NaClO disinfection 15 min.The most suitable base medium for ger-mination is 1/2MS.The most favorable hormone combination for seed germination of M.chinghaiensis was 1/2 MS+6-BA 2 mg/L+NAA 0.1 mg/L+ GA3 0.3 mg/L.[Conclusion]By exploring the germination system of M.chinghaiensis seeds,this study can alleviate the bottleneck problem that M.chinghaiensis seed-lings are extremely difficult to obtain for artificial propagation,and provide new support for further research on its pharmacological value and biological properties.
关键词
青海茄参/种子萌发/低温处理/组织培养/激素组合Key words
Mandragora chinghaiensis/seed germination/low temperature treatment/tissue culture/hormone combination引用本文复制引用
基金项目
国家自然科学基金项目(31860575)
青海省科学技术厅自然科学基金项目(2017-ZJ-923Q)
青海省林业厅林木种苗总站国家长期科研基地监测项目(2130211)
出版年
2024
NETL
NSTL
万方数据