Expression and distribution of THRs in oviduct and uterus of Ganjia Tibetan sheep during estrus cycle
[Objective]The study aimed to elucidate the expressions of Thyroid hormone receptor α(THRα)and Thyroid hormone receptor β(THRβ)in the oviduct and uterus during the estrous cycle of Ovis aries,exploring their regulation effect on breeding activities of the Tibetan sheep.[Method]Thirty healthy and non-pregnant Ganjia Tibetan sheep in estrus and anestrus were employed.Real-time PCR,Western blot,and immunohistochemical staining were used to study the expression and protein content of THRα and THRβ mRNA in the oviduct and uterine tissues during estrus and anestrus.[Result]The THRα and THRβ mRNA and their proteins were expressed in the fallopian tubes and uterus of Tibetan sheep during the es-trus cycle and anestrus period.The expression levels of THRα and THRβ mRNA in the fallopian tubes were the highest in the late estrus period,which were significantly(P<0.05)higher than those in the other three periods of the estrus cycle and anestrus period.The average relative expression level of THRβ mRNA was significantly higher than that of THRα mRNA in utero during estrus(P<0.05).The expression level of THRα protein in oviduct tissues was the highest in the late estrus period,and the highest in the uterus tis-sues in estrus period,which was significantly(P<0.05)higher than was in other periods.The expression level of THRβ protein in the oviduct and uterus was the highest in late estrus,which significantly differed from the other periods(P<0.05).The average expression levels of THRα and THRβ mRNA and their pro-tein in the anestrous period were significantly lower than those in each estrous period.The results of immu-nohistochemical staining showed that THRα and THRβ immunoreactive cells were mainly expressed in the mucosal epithelium,ciliary cells,secretory cells,and myometria of the fallopian tube,and in stromal cells and uterine glands of the uterus.[Conclusion]The different-concentration expression of THRα and THRβ suggested that THRs could regulate the fallopian tube and uterus of Ganjia Tibetan sheep during estrus,which was probably involved in the reproductive physiological activities of Ganjia Tibetan sheep.
NETL
NSTL
万方数据
维普
