Determination of the gender differentiation stage of chicken in the process of embryonic development
[Objective]This study aims to determine the period of sex differentiation during the incuba-tion of chicken embryos.[Method]The research subject was the yellow feather broiler.The incubation con-ditions for fertilized eggs were set at a temperature of 37.8℃and a humidity of 60%RH.The egg shape in-dex method,embryo line observation,gonad organ morphology,gonad histology observation,and molecular biology identification were utilized to ascertain the stage of sex differentiation in the developing embryos.The experiment was conducted three times.[Result]Using the embryo line observation method,it was found that by the third day of incubation,male and female embryos could be distinguished based on the thick-ness and shape of blood vessel branches.Morphological examination of gonadal organs on the eighth day re-vealed that male gonads developed symmetrically,whereas female gonads developed asymmetrically.Histo-logical observation of the gonads from seven to ten days of incubation indicated that female gonads exhib-ited a cortex and medulla structure,whereas male gonads displayed a structure resembling convoluted semi-niferous tubules.The initial determination of the sex differentiation stage in chicken embryos was identified as occurring on the seventh day of development.Subsequent molecular biology testing showed a single am-plification band in males,approximately 550 base pairs(bp)in size,between three to ten days of incubation.In contrast,females displayed two amplification bands,sized at 550 and 450 bp.Therefore,the sex differen-tiation stage,as determined by molecular biology methods,may be identified as early as the third day of incu-bation.[Conclusion]The sex differentiation of chicken embryos occurs between the third and eighth days of embryonic incubation.
NETL
NSTL
万方数据
维普
