Analysis of fecal microbial diversity in a type Ⅱ diabetes mouse model
[Objective]To elucidate the role and influence of fecal microorganisms on type Ⅱ diabetes mellitus(T2DM).[Method]In this study,SPF-grade BKS-DB male mice served as models for T2DM.The community structure and composition of fecal microbial 16S rRNA genes were analyzed in both the control group(CN group)and the T2DM model group(T2DM group).[Result]At the phylum level,the abundance of Patescibacteria and Campilobacterota in the feces of mice in the T2DM group was signifi-cantly higher than that in the CN group.At the genus level,16 endemic genera were identified in the T2DM group,with Kurthia being the most abundant.Among the top 10 genera with significant differences,the abundance of flora in 9 genera in the T2DM group was significantly higher than in the CN group(P<0.05),while the abundance of microbiota in 1 genus in the T2DM group was significantly lower.LEfSe analysis re-vealed that the genera Anaerofustis and Corynebacterium were enriched in the T2DM group and are both as-sociated with immunocompromised individuals,showing a positive correlation with diabetes.[Conclusion]In the feces of T2DM model mice,high expression of flora such as Patescibacteria and Bacteroidota was ob-served,along with an imbalance between the genera Anaerofustis and Corynebacterium.This imbalance may contribute to decreased immune function and positively influence the development of diabetes.
type 2 diabetesmodel mice with type 2 diabetesfecal microorganisms
NETL
NSTL
万方数据
维普
