Alleviating effects of salicylic acid and 5-aminolevulinic acid on maize seedlings under salt stress
[Objective]The mitigating effects of different concentrations of exogenous salicylic acid(SA),5-aminolevulinic acid(5-ALA)and their optimal concentration on maize seedlings under salt stress were investigated.[Method]The salt-sensitive inbred line PH4CV was used as the experimental material.Under the condition of 180 mmol/L salt stress,different concentrations of SA,5-ALA treatment solution and their optimal concentration composite treatment solution were added to determine the seedling length,root length,aboveground fresh weight and underground fresh weight of maize seedlings,and the activities of catalase(CAT),superoxide dismutase(SOD)and peroxidase(POD)in leaves and roots.[Result]Under salt stress,when exogenous SA and 5-ALA were applied alone,with increasing concentration,seedling length,root length,aboveground fresh weight,underground fresh weight of maize seedlings and CAT,SOD and POD activities of leaves and roots of maize seedlings first increased and then decreased.Excessive con-centration would affect the growth anddevelopment of maize seedlings.When the optimal concentration of SA and 5-ALA was applied,the phenotypic and physiological characteristics of maize seedlings showed an increasing trend,and the mitigating effect was better than the two hormones alone.[Conclusion]The multi-index fuzzy membership function method was used for comprehensive evaluation.When the concentration of SA and 5-ALA was 0.5 mmol/L SA and 50 mg/L 5-ALA,and the combination ratio of the two hor-mones was 1/2(50 mg/L 5-ALA)+ 0.5 mmol/L SA,the effect of alleviating salt stress in maize seed-lings was the best.